丹皮酚磺酸钠对免疫性结膜炎的治疗作用及机制

目的：探讨丹皮酚磺酸钠对小鼠实验性免疫性结膜炎的治疗作用及机制。方法：实验研究。采用随机数字表法将36只（72眼）Balb/c小鼠平均分为正常对照组（A组）、模型组（B组）、1%丹皮酚磺酸钠组（C组）、2%丹皮酚磺酸钠组（D组）、色甘酸钠滴眼液对照组（E组）、奥洛他定滴眼液对照组（F组），每组6只。以鸡卵白蛋白（OVA）腹腔注射免疫B、C、D、E、F组建立免疫性结膜炎动物模型；7 d后，A组和B组予磷酸缓冲盐溶液（PBS）滴眼，C、D组分别予1%、2%丹皮酚磺酸钠滴眼治疗，E、F组分别予色甘酸钠、奥洛他定滴眼液治疗。用药5 d，最后1次用药后进行眼部临床症状评分。以甲苯胺蓝染色观察检测结膜组织切片肥大细胞脱颗粒（MCD）情况，酶联免疫吸附测定法（ELISA）检测各组小鼠血清的卵蛋白特异性免疫球蛋白E（OVA-s IgE）、干扰素γ（IFN-γ）及白介素4（IL-4）水平。所得数据组间比较采用单因素方差分析。结果：各组间症状评分差异具有统计学意义（F=38.72，P<0.001），其中B组症状评分明显高于A组（P<0.001），C、D、E、F组的症状评分均低于B组（均P<0.05）。各组间肥大细胞计数差异具有统计学意义（F=15.28，P<0.001），B组及各用药组肥大细胞脱颗粒计数均明显高于A组（均P<0.05）。C、D、E、F组小鼠血清中OVA-s IgE水平明显低于B组（P<0.05）；A、C、D、E、F组小鼠血清中IFN-γ水平均明显高于B组（P<0.05），IL-4水平均明显低于B组（P<0.05）。结论：丹皮酚磺酸钠对实验性免疫性结膜炎具有治疗作用，能够稳定实验性免疫性结膜炎小鼠眼结膜组织中肥大细胞，抑制活化脱颗粒，下调小鼠血清中OVA-sIgE、IL-4水平及上调IFN-γ水平。

The Therapeutic Effect and Mechanism of Sodium Paeonolsilate on Immunologic conjunctivitis

Objective: To investigate the therapeutic effect of sodium paeonolsilate on experimental immunologic conjunctivitis in mice and to explore the mechanism of action. Methods: This was an experimental study.The Balb/c mice which immunologic conjunctivitis was initiated by intraperitoneal injection with ovalbumin (OVA) were randomly assigned to five treatment groups (Groups B-F, n=6 mice each), and another 6 mice served as uninjected controls (Group A). Both eyes of each mouse were treated with eyedrops 7 days after as follows: Group A, uninjected controls treated with phosphate-buffered saline (PBS); Group B, model group treated with PBS; Group C, treated with 1% sodium paeonolsilate group;Group D, treated with 2% sodium paeonolsilate group; Group E, treated with sodium cromoglicate;Group F, treated with olopatadine. After five days of medication or control treatments, ocular clinical symptoms were evaluated, and blood and conjunctival tissue were taken for analysis. Conjunctival tissue sections were stained with toluidine blue to visualize mast cell degranulation (MCD). Ovalbumin specific immunoglobulin E (OVA-s IgE), interferon-γ (IFN-γ), and interleukin-4 (IL-4) levels were determined in serum by enzyme linked immunosorbent assay. One-way analysis of variance was used for the comparison of data among the groups. results: Symptom scores were significant among all groups (F=38.72, P<0.001). The symptom scores in Group B, were significantly higher than those in Group A (P<0.001).The symptom scores in each medication group were lower than those in Group B (P<0.05). There were significant differences in the mast cell among the six groups (F=15.28, P<0.001). The number of mast cell higher in Groups B-F than in the group A (all P<0.01). The serum levels of OVA-s IgE were significantly lower in Groups C-F than in the Group B (all P<0.05). The serum IFN-γ levels were significantly higher in Group A and Groups C-F than in Group B (all P<0.05). The serum IL-4 levels were significantly lower in Group A and Groups C-F than in Group B (all P<0.05). conclusions: In mice, sodium paeonolsilate eye drops have a therapeutic effect on experimental immunologic conjunctivitis initiated by OVA injection.It stabilizes mast cells and inhibits activated degranulation. Sodium paeonolsilate down regulated serum levels of OVA-s IgE and IL-4 while upregulating IFN-γ.