丙氨酸氨基转移酶测定波长设置的实验研究

目的观察波长设置对含有不同干扰物丙氨酸氨基转移酶（ALT）标本测定的影响。方法对NADH纯品溶液、试剂样本及含不同干扰物的样品进行全波长扫描。依据吸收曲线设定不同的副波长,然后对含不同干扰物的定值血清进行测定,以定值血清所给的值为参照,确定最佳波长组合。结果定值血清测量结果,单波长340nm与双波长（340/380nm,340/404nm）差异无统计学意义（P〉0.05）;血红蛋白（Hb）和胆红素（BIL）干扰的ALT标本,340/404nm测定结果与靶值差异无统计学意义（P〉0.05）,340/380nm测定结果与靶值差异有统计学意义（tHb=2.144,PHb=0.042〈0.05;tBIL=2.453,PBIL〈0.05）;血脂（TG）干扰的ALT标本340/380nm测定结果与靶值差异无统计学意义（P〉0.05）,而340/404nm测定结果与靶值差异有统计学意义（t=-3.338,P〈0.01）。结论利用单波长即可准确测得低干扰程度丙氨酸氨基转移酶（ALT）标本的活性。若采用双波长时应视标本干扰物的种类而选择不同双波长,溶血、黄疸干扰应选择340/404nm,血脂干扰应选择340/380nm。

Studies on selecting wavelengths during alanine aminotransferase determination

Objective To observe the effects of selecting wavelengths on alanine aminotransferase (ALT ) determination for samples with different interferences.Methods Full-wavelengths scanning was carried out on NADH standard solution, regents and samples with different interferences. The second wavelenghs were selected based on the absorbance curve. The ALT of standard serum containing different interferences was measured. The suitable combination of wavelenghs was decided.Results There was no remarkable difference between single wavelength (340 nm) and dual wavelengths (340/380 nm, 340/404 nm) (t < 2.064,P>0.05). If two wavelengths were selected, the results of determination of ALT containing hemoglobin and total bilirubin interferences,taking 340/404 nm, are no remarkable differences with target value (P>0.05). But with 340/380 nm, there was a significant difference (tHb= 2.144,PHb=0.042<0.05;tBIL= 2.453,PBIL= 0.022<0.05). To ALT samples containing high triglycerides, there was no remarkable difference between the results of determination and target values with 340/380 nm. But with 340/404 nm, the significant difference had been shown (t=-3.338,P= 0.003< 0.05).Conclusion With single wavelength(340 nm), the exact results of ALT activity can be acquired. If two wavelengths taken, proper wavelengths should be selected according to different interferences. 340/404 nm is fit for the detemination of ALT samples containing hemoglobin and total bilirubin, but with triglycerides interferences, 340/380 nm is more proper.