Toll样受体7、8、9在原发性舍格伦综合征中的表达

目的:检测原发性舍格伦综合征(primary Sjogren's syndrome,pSS)患者血细胞中Toll样受体(TLR)7、8、9的表达水平,并检测TLR7、9在pSS患者腮腺组织中的表达.方法:采用实时定量PCR,对37例pSS患者和24例对照组患者血细胞中TLR7、8、9的水平进行检测,分析2组之间的差异.应用免疫荧光法和免疫组化分别检测TLR7和TLR9在pSS患者腮腺组织中的表达.应用SAS6.12软件包,对数据进行t检验.结果:实时定量PCR显示,pSS组的TLR7、9的mRNA表达量显著高于对照组,2组之间有显著差异(P<0.05),pSS组的TLR7是对照组的2.17倍,pSS组的TLR9是对照组的2.33倍.而TLR8在2组之间无显著差异(p>0.05).免疫荧光和免疫组化发现,TLR7、9在pSS组患者腮腺组织中的导管上皮细胞、淋巴细胞和上皮岛均为阳性;而对照组腮腺组织中仅导管上皮细胞为阳性.结论:TLR7和TLR9在原发性舍格伦综合征患者中存在表达异常.

xpression of Toll-like receptors 7,8,and 9 in primary Sj(o)gren's syndrome

PURPOSE： The purpose of this study was to investigate the level of Toll-like receptors（TLRs） 7,8 and 9 in peripheral blood cells from patients with primary Sjogren＇s syndrome（pSS） and whether TLR7 and 9 exist in the parotid glands of pSS.METHODS： Peripheral blood cells and parotid gland biopsid specimens from patients with pSS were studied.TLR7,TLR8 and TLR9 gene mRNA levels in peripheral blood cells were determined by using real-time PCR.TLR7-and TLR9-positive cells in parotid glands from 3 pSS patients and 3 controls were observed by using immunofluorescence and immunohistochemistry,respectively.Statistical analysis was performed by Student＇s t test using SAS 6.12 software package.RESULTS： TLR7 and TLr9 mRNA levels were 2.17-and 2.33-fold higher in pSS patients than control subjects,respectively（P0.05）,whereas TLR8 levels were not different between the two groups（P0.05）.TLR7-and TLR9-positive cells of the pSS group were localized in the epithelial islands,lymphocytes,and ductal epithelial cells of the parotid glands and were more abundant than the TLR7-and TLR9-positive cells that were only localized in the ductal epithelial cells of parotid glands of control subjects.CONCLUSIONS： TLR7 and TLR9 mRNA levels are up-regulated in the peripheral blood of pSS patients,and TLR7-and TLR9-positive cells exist in the epithelial islands,lymphocytes,and ductal epithelial cells of the parotid glands of individuals affected by pSS,but are limited to the ductal epithelial cells of controls.Supported by Research Fund of Science and Technology Commission of Shanghai Municipality（08DZ271100） and Shanghai Leading Academic Discipline Project（S30206）.