| SARS冠状病毒S蛋白抗原表位串联基因的设计和表达 |
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| 引用本文： | 房凯,钟连声,赵雨杰. SARS冠状病毒S蛋白抗原表位串联基因的设计和表达[J]. 中国医科大学学报, 2007, 36(1): 33-34 |
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| 作者姓名： | 房凯 钟连声 赵雨杰 |
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| 作者单位： | 中国医科大学基础医学院生物信息学教研室,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省科技厅科技计划基金 |
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| 摘 要： | 目的:表达严重急性呼吸综合征(SARS)冠状病毒S蛋白的抗原表位序列,为研制新型的SARS病毒疫苗提供新思路。方法:应用生物信息学分析软件分析SARS冠状病毒S蛋白的抗原表位序列,设计全新的抗原表位编码基因序列,构建表达载体并在大肠杆菌中表达该基因。结果:设计并合成了SARS冠状病毒S蛋白的抗原表位序列的新基因序列,在大肠杆菌中实现了该基因的高表达。结论:可以重新设计新基因产生新型候选重组疫苗。
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| 关 键 词： | 严重急性呼吸综合征 抗原表位 生物信息学 S蛋白 |
| 文章编号： | 0258-4646(2007)01-0033-02 |
| 修稿时间： | 2006-06-21 |
Design and expression of antigen epitope of spike protein of severe acute respitary syndrome coronavirus |
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| FANG Kai,ZHONG Lian-sheng,ZHAO Yu-jie. Design and expression of antigen epitope of spike protein of severe acute respitary syndrome coronavirus[J]. Journal of China Medical University, 2007, 36(1): 33-34 |
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| Authors: | FANG Kai ZHONG Lian-sheng ZHAO Yu-jie |
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| Affiliation: | Department of Bioinfonnatics, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China |
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| Abstract: | Objective:To design a new gene encoding the antigen epitope of the spike protein(S protein) of severe acute respiratory syndrome coronavirus (SARS-CoV) and express the new gene. Methods:By analyzing the antigen epitope of SARS-CoV S protein with antigenic peptides prediction software,a new gene was designed,and its expression vector was constructed. Then the gene was expressed in Escherichia coli (E coli). Results:The new gene encoding the antigen epitope of SARS-CoV S protein was designed and synthesized successfully,and it was highly expressed in E coli. Conclusion:The new gene encoding the antigen epitope of SARS-CoV S protein could be designed,which provides basis for the development of the new type of recombinant vaccine candidate. |
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| Keywords: | severe acute respiratory syndrome epitope bioinformatics spike protein |
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