| D-半乳糖衰老大鼠睾丸生精细胞端粒酶表达的变化 |
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| 引用本文： | 陈志宏,杨国军. D-半乳糖衰老大鼠睾丸生精细胞端粒酶表达的变化[J]. 承德医学院学报, 2006, 23(2): 109-111 |
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| 作者姓名： | 陈志宏 杨国军 |
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| 作者单位： | 承德医学院,河北,承德,067000;承德医学院,河北,承德,067000 |
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| 基金项目： | 河北省承德市科技局资助项目 |
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| 摘 要： | 目的:观察D-半乳糖衰老大鼠血清睾酮含量及睾丸生精细胞端粒酶表达的变化.方法:20只SD大鼠随机分为正常组、衰老模型组,每组均为10只.采用D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型.ELISA方法检测各组大鼠血清睾酮含量、SP免疫组化法检测睾丸生精细胞端粒酶的表达.结果:衰老大鼠血清睾酮含量明显下降(与正常大鼠相比P＜0.05);衰老大鼠睾丸生精细胞端粒酶的表达明显少于正常大鼠.结论:衰老大鼠睾丸生精细胞增殖能力下降,睾丸功能减退.
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| 关 键 词： | 衰老 睾酮 端粒酶 |
| 文章编号： | 1004-6879(2006)02-0109-03 |
| 修稿时间： | 2006-01-03 |
STUDY ON THE CHANGES OF TELOMERASE EXPRESSION OF SPERMATOGENIC CELLS IN TESTIS OF D-GAL AGING RATS |
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| CHEN Zhi-hong,YANG Guo-jun. STUDY ON THE CHANGES OF TELOMERASE EXPRESSION OF SPERMATOGENIC CELLS IN TESTIS OF D-GAL AGING RATS[J]. Journal of Chengde Medical College, 2006, 23(2): 109-111 |
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| Authors: | CHEN Zhi-hong YANG Guo-jun |
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| Abstract: | Objective: To observe the changes of testosterone content in serum and telomerase expression of spermatogenic cells in testis of D-gal aging rats.Methods: 20 SD rats were applied in this study and divided into 2 groups at random: normal group,aging model group,and each group had 10 rats. The sub-acutely aging model rats were made by injecting D-gal into abdominal cavity continually.ELISA was used to detect the testosterone content in serum,SP immunohistochemistry to observe the telomerase expression of spermatogenic cells.Results: The testosterone content of aging rats decreased significantly compared with normal rats(P<0.05);the telomerase positive cells of aging rats were much less than normal rats.Conclusions: The reproductive activity of spermatogenic cells decreased in aging rats,and the function of testis decline. |
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| Keywords: | Aging Testosterone Telomerase |
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