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| DPPH法测定九州虫草不同极性部位抗氧化活性 | |
| 引用本文： | 董秀英,吕青涛,张国英,凌建亚,张长铠. DPPH法测定九州虫草不同极性部位抗氧化活性[J]. 中国实验方剂学杂志, 2011, 17(10): 70-73 |
| 作者姓名： | 董秀英 吕青涛 张国英 凌建亚 张长铠 |
| 作者单位： | 1. 山东大学微生物技术国家重点实验室,济南,250100;山东中医药大学药学院,济南,250355 2. 山东中医药大学药学院,济南,250355 3. 山东大学微生物技术国家重点实验室,济南,250100 |
| 基金项目： | 国家自然基金面上项目，山东省优秀中青年科学家奖励基金 |
| 摘 要： | 目的:研究九州虫草体外不同极性部位的抗氧化作用。方法:采用DPPH法测定九州虫草石油醚(EVP)、乙酸乙酯(EVE)、正丁醇(EVB)和水(EVW)不同提取部位的抗氧化活性。结果:EVP,EVE,EVB,EVW均有DPPH自由基的清除能力,测得EVP,EVE,EVB和EVW清除DPPH的EC50值分别为190.2,141.4,151.6,195.4 mg·L-1。结论:九州虫草具有很强的抗氧化性,为进一步研究其抗氧化活性成分提供了科学依据。 |
| 关 键 词： | 九州虫草 1,1-二苯基-2-苦基苯肼自由基法 体外抗氧化作用 |
| 收稿时间： | 2011-01-12 |
Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay in vitro |
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| DONG Xiu-ying,LV Qing-tao,ZHANG Guo-ying,LING Jian-ya and ZHANG Chang-kai. Determination of the Antioxidant Capacity of Cordyceps Kyushuensis with DPPH Assay in vitro[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(10): 70-73 | |
| Authors: | DONG Xiu-ying LV Qing-tao ZHANG Guo-ying LING Jian-ya ZHANG Chang-kai |
| Affiliation: | State Key Laboratory of Microbial Technology of Shandong University, Jinan 250100, China; College of Pharmacology of Shandong University of Traditional Chinese Medicine, Jinan 250355,China;College of Pharmacology of Shandong University of Traditional Chinese Medicine, Jinan 250355,China;State Key Laboratory of Microbial Technology of Shandong University, Jinan 250100, China; College of Pharmacology of Shandong University of Traditional Chinese Medicine, Jinan 250355,China;State Key Laboratory of Microbial Technology of Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology of Shandong University, Jinan 250100, China |
| Abstract: | Objective: To investigate the antioxidant effect in vitro of Cordyceps kyushuensis. Method :Antioxidant capacity of the petroleum ether fraction(EVP);acetic ether fraction(EVE);n-butanol fraction(EVB)and water fraction(EVW)were determined using the DPPH assay in vitro. Result: EVP,EVE,EVB and EVW showed antioxidant capacity of eliminating DPPH free radicals,and the scavenging efficiency increase with the concentration increase. EC50 values of the EVP,EVE,EVB and EVW are 190.2,141.4,151.6,195.4 mg ·L-1 respectively. Conclusion: C. kyushuensis shows great oxidation resistance,and the results of the antioxidant effect of the EVP,EVE,EVB, EVW provide a scientific basis to the research of active constituent and the exploitation of C.kyushuensis. |
| Keywords: | Cordyceps kyushuensis DPPH antioxidant capacity |
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