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| 理中汤对Caco-2细胞PepT1转运功能的影响 | |
| 引用本文： | 郭文峰,杨伟鹏,王怡薇,王彦礼,胡灿,温鹏,高小玲,陈蔚文.理中汤对Caco-2细胞PepT1转运功能的影响[J].中国实验方剂学杂志,2011,17(10):108-111. |
| 作者姓名： | 郭文峰 杨伟鹏 王怡薇 王彦礼 胡灿 温鹏 高小玲 陈蔚文 |
| 作者单位： | 1. 广州中医药大学脾胃研究所,广州,510405 2. 中国中医科学院中药研究所,北京,100700 3. 广州中医药大学脾胃研究所,广州,510405;上海市高校中医内科学E-研究院,上海中医药大学,上海,201203 |
| 基金项目： | 国家自然科学基金项目，上海市教育委员会E-研究院建设计划项目 |
| 摘 要： | 目的:观察理中汤对Caco-2细胞肽转运载体(PepT1)转运二肽的能力及其蛋白、mRNA表达的影响。方法:Caco-2细胞融合后连续培养28 d后给予理中汤处理,并设立空白及cAMP抑制剂对照组,用放射性同位素示踪技术比较Caco-2细胞转运二肽化合物Glycyl-Sarcosine的能力,用Western blot法测定Caco-2细胞膜上PepT1蛋白的表达,荧光定量PCR法测定PepT1 mRNA(SLC15A1)的表达水平。结果:理中汤处理组Caco-2细胞15,30,60,120 min时点Glycyl-Sarcosine吸收转运累计量分别为(4.52±0.57),(6.34±0.57),(7.29±0.64),(8.23±0.67)μmol/孔,高于空白对照组(P<0.05),后者分别为(2.42±0.68),(3.15±0.76),(5.62±0.20),(6.54±0.54)μmol/孔;理中汤与Rp-8-Br-cAMP合用组15,30,120 min时点分别为(2.68±0.46),(4.13±0.46),(6.73±0.43)μmol/孔,明显低于相应时点单用理中汤组(P<0.05);理中汤处理后Caco-2细胞膜PepT1蛋白表达增加,PepT1表达与内参GAPDH表达灰度比值理中汤组为(0.326±0.031),高于空白对照组(0.220±0.019)(P<0.01);理中汤对Caco-2细胞SLC15A1 mRNA表达未见明显作用,但与cAMP抑制剂合用,理中汤能逆转Rp-8-Br-cAMP下调Caco-2细胞SLC15A1 mRNA表达的作用。结论:理中汤具有促进正常培养Caco-2细胞转运二肽化合物Glycyl-Sarcosine的作用,该作用可能与其促进胞浆中PepT1蛋白嵌入胞膜中发挥转运二肽的功能有关,该过程调控与胞内第二信使cAMP有一定的关系。 |
| 关 键 词： | 理中汤 肽转运载体1 转运功能 环磷酸腺苷 |
| 收稿时间： | 1/4/2011 12:00:00 AM |
Effects of Lizhong Decoction on the Transport Ability of PepT1 in Caco-2 Cells |
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| GUO Wen-feng,YANG Wei-peng,WANG Yi-wei,WANG Yan-li,HU Can,WENG Peng,GAO Xiao-ling and CHEN Wei-wen.Effects of Lizhong Decoction on the Transport Ability of PepT1 in Caco-2 Cells[J].China Journal of Experimental Traditional Medical Formulae,2011,17(10):108-111. | |
| Authors: | GUO Wen-feng YANG Wei-peng WANG Yi-wei WANG Yan-li HU Can WENG Peng GAO Xiao-ling and CHEN Wei-wen |
| Institution: | Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; E-Institute of Shanghai Municipal Education Commission, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
| Abstract: | Objective:To observe the effects of Lizhong Decoction on expression and transport ability of PepT1 in Caco-2 cells.Method:Caco2 monolayers were grown on permeable supports.Peptide transport activity was studied using -glycyl-sarcosine(-Gly-Sar).The densities of PepT1 protein and mRNA(SLC15A1)expression levels were analyzed by Western blot and real-time quantitative Polymerase Chain Reaction.Result: The total transported Gly-Sar of Caco-2 cells within 15,30,60,120 min of group treated by Lizhong Decoction we... |
| Keywords: | Lizhong Decoction peptide transporter 1 transport ability cyclic adenosine monophosphate |
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