锌转运体ZNT1在人阿尔茨海默病大脑的定位

目的研究锌转运体-1(zinc transporter 1,ZNT1)在阿尔茨海默病(Alzheimer's Disease,AD)尸检大脑皮层内的定位分布,探讨ZNT1影响脑锌平衡从而参与AD发病的可能机制。方法应用免疫组织化学、免疫荧光和共聚焦激光扫描显微镜观察ZNT1蛋白和β-淀粉样蛋白(β-amyloid,Aβ)在AD患者大脑内的分布和二者之间的位置关系。结果ZNT1免疫阳性反应产物主要定位于AD大脑皮层老年斑内。几乎所有的Aβ老年斑内均有不同程度ZNT1表达。ZNT1和β-淀粉样蛋白免疫双标结果进一步证实ZNT1免疫产物与Aβ共存于AD患者的老年斑内。结论AD患者大脑皮质Aβ老年斑内有大量ZNT1蛋白表达,提示ZNT1可能参与AD大脑皮质Aβ老年斑的形成。

The Localization of ZincTransporter1(ZNT1) in Alzheimer's Disease Brain

Objective To investigate the localization of ZNT1 in Alzheimer's diseased (AD)brain, and to explore the possible role of ZNT1 in AD pathogenesis. Methods Immunohistochemistry, immunofluorescence and confocal laser scanning microscopy were used to analyze the distributions of ZNT1 and β-amyloid protein(Aβ)and the relationship between these two proteins in AD brain. Results ZNT1 immunoreactive product was seen to be localized in the senile plaques within the cerebral cortex of AD patients.Almost all of Aβ senile plaques presented ZNT1 immunoreactivity with different expression levels.Confocal microscopic results revealed that a colocalization of ZNT1 and Aβ was found in the senile plaques. Conclusion The abundant expression of ZNT1 in Aβ plaques suggests that ZNT1 may be involved in the formation of Aβ senile plaque in AD brain.