胰岛素样因子3在邻苯二甲酸二（2-乙基）己酯诱导隐睾发生过程中的表达分析

目的：研究环境内分泌干扰物邻苯二甲酸二（2-乙已基）酯（Di-（2-ethylhexyl）phthalate,DEHP）作用于孕鼠后,其胎鼠睾丸组织中胰岛素样因子3（Insulin like factor3,INSL3）mRNA和蛋白质的表达情况;探讨DEHP诱发小鼠隐睾的分子机制.方法：妊娠KM小鼠随机分为3组：正常对照组、玉米油对照组和DEHP实验组.玉米油对照组和DEHP实验组自妊娠12.5d到妊娠18d分别持续经口予以玉米油和DEHP500mg/（kg.d）,正常对照组不予灌药.结果：正常对照组和玉米油对照组睾丸组织中INSL3 mRNA和蛋白质的表达均无差异（P〉0.05）;DEHP实验组INSL3 mRNA的表达量约为对照组的1/3,与对照组相比其差异具有显著性（P〈0.05）;实验组INSL3蛋白质的表达量约为对照组的1/4,与对照组相比其差异具有显著性（P〈0.05）.结论：环境内分泌干扰物DEHP下调睾丸间质细胞INSL3 mRNA和蛋白质的表达,影响睾丸引带发育,可能是DEHP诱发隐睾的分子机制之一.

Effect of Di-(2-ethylhexyl) phthalate on insulin like factor 3 expression in fetal mice

AIM：To investigate the expression of Insulin like factor 3（INSL3）in fetal mice exposed to Di-（2-ethylhexyl）phthalate（DEHP）in pregnancy.METHODS：KM mice were divided into 3 groups randomly：normal control group,corn oil group and DEHP-exposed group.The last two groups were administrated respectively with corn oil and DEHP 500 mg/（kg·d）to the pregnant mice from gestation day 12.5（GD12.5）to GD18,and normal group with nothing.RESULTS：Expression of INSL3 mRNA and protein in normal and corn group were no significance（P〉0.05）.Expression of INSL3 mRNA in DEHP-exposed group was about one-third of that in normal group,which was significantly decreased（P〈0.05）.Similarly,expression of INSL3 protein in DEHP-exposed group was about quarter of that in normal group,which was also significantly decreased（P〈0.05）.CONCLUSION：DEHP as one of environmental endocrine disruptors,can down-regulate the mRNA and protein expression of INSL3 and interfere the gubernaculum development.It may be a possible mechanism that DEHP causes cryptorchidism.