| CD14在B1细胞的表达研究 |
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| 引用本文： | 马小莹,高继鑫,李巍,付萌,安金刚,顾伟杰,刘玉峰. CD14在B1细胞的表达研究[J]. 医学争鸣, 2009, 0(19): 1843-1845 |
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| 作者姓名： | 马小莹 高继鑫 李巍 付萌 安金刚 顾伟杰 刘玉峰 |
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| 作者单位： | 第四军医大学西京医院全军皮肤病研究所; |
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| 基金项目： | 国家自然科学基金(30872299) |
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| 摘 要： | 目的：比较分析小鼠B1细胞和B2细胞CD14的表达,探索B1细胞在天然免疫中的作用.方法：取正常C57BL/6小鼠的腹腔和脾细胞,anti-IgM免疫磁珠分选B1和B2细胞,提取总RNA后用半定量逆转录聚合酶链式反应（RT-PCR）检测CD14 mRNA的表达,以anti-CD14抗体、anti-CD19抗体流式细胞仪分析CD14分子在B1和B2细胞表面的表达;进一步给予小鼠腹腔内注射脂多糖（LPS）,于不同时间点分选B1细胞,RT-PCR检测CD14 mRNA表达水平的变化.结果：RT-PCR及流式细胞仪检测结果显示CD14在正常小鼠B1细胞有较低水平的表达,而B2细胞几乎没有表达,LPS刺激后B1细胞的CD14表达水平明显升高.结论：CD14表达于正常小鼠B1细胞,并且可能在B1细胞对LPS的应答中具有重要作用.
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| 关 键 词： | B1细胞 CD14 LPS |
CD14 expression on B-1 lymphocyte |
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| MA Xiao-Ying,GAO Ji-Xin,LI Wei,FU Meng,AN Jin-Gang,GU Wei-Jie,LIU Yu-FengPLA Institute of Dermatology,Xijing Hospital,Fourth Military Medical University,Xi'an ,China. CD14 expression on B-1 lymphocyte[J]. Negative, 2009, 0(19): 1843-1845 |
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| Authors: | MA Xiao-Ying GAO Ji-Xin LI Wei FU Meng AN Jin-Gang GU Wei-Jie LIU Yu-FengPLA Institute of Dermatology Xijing Hospital Fourth Military Medical University Xi'an China |
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| Affiliation: | MA Xiao-Ying,GAO Ji-Xin,LI Wei,FU Meng,AN Jin-Gang,GU Wei-Jie,LIU Yu-FengPLA Institute of Dermatology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM:To further observe B-1 B cell function in innate immune system,we compare and analyze CD14 expression on B1 and B2 cells.METHODS:Peritoneal cells and splenocytes of normal C57BL/6 mice were isolated and purified with anti-IgM magnetic beads.The total RNA of the cells were extracted and the expression level of CD14 was detected by semiquantitative RT-PCR.CD14 expression on B1 and B2 cells were measured using anti-CD14 and anti-CD19 antibodies by flow cytometry(FCM).Furthermore,LPS was injected into mice ... |
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| Keywords: | B1 cell CD14 LPS |
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