慢性乙型肝炎患者外周血单核细胞TLR7mRNA表达与血清IL-12，HBV-DNA载量的相关性

目的：研究TLR7在慢性乙型肝炎患者外周血单核细胞的表达及其与IL-12，HBV—DNA载量之间的关系，探讨TLR7在抗病毒免疫应答中的作用机制及意义．方法-用实时荧光定量PCR法检测38例具有不同HBV-DNA载量的慢性乙型肝炎患者和12例正常对照者外周血单核细胞TLR7mR-NA表达，患者按病毒载量分为低、中、高3组．实时荧光定量PCR法测定血清病毒载量，ELISA法测定血清IL-12的水平，多组均数间的比较用方差分析，直线相关性分析用Spearman秩相关．结果：患者组中TLR7mRNA水平比正常对照组显著降低（P〈0．01），3组患者间TLR7mRNA表达有显著差异且与血清HBV—DNA载量呈负相关，患者组血清IL-12比正常人显著增高，3组患者外周血清IL-12，低、中病毒载量组的差异无统计学意义（P〉0．05），其余各组间差异有统计学意义（P〈0．01），且与病毒载量呈负相关（r=-0．075，P〈0．01）．结论：TLR7在慢性乙型肝炎患者外周血单核细胞的表达下降，TLR7与IL-12可能在慢性乙型肝炎中对病毒清除有重要作用，其水平降低可能与疾病的慢性化机制有关．

Correlation between expression of TLR7 mRNA in peripheral blood monouclear cells of chronic hepatitis B patients and IL-12,HBV-DNA load

AIM:To investigate the correlation between the expression of TLR7 mRNA in peripheral blood monouclear cells(PBMCs) of chronic hepatitis B patients and IL-12 and HBV-DNA load and to explore the role of TLR7 in anti-HBV-DNA.METHODS:TLR7 mRNA expression was examined by real-time quantitative PCR in 38 patients with different HBV-DNA loads and 12 healthy controls,and the patients were divided into 3 groups based on low,medium or high viral load.HBV-DNA was examined by real-time quantitative PCR and the level of...