| APC-Cdh1在中枢神经系统中的表达分布特点 |
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| 引用本文： | 姚文龙,张传汉,钱巍,邱瑾,柳璐,祝畅,桂伶俐.APC-Cdh1在中枢神经系统中的表达分布特点[J].第四军医大学学报,2009(13):1161-1164. |
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| 作者姓名： | 姚文龙 张传汉 钱巍 邱瑾 柳璐 祝畅 桂伶俐 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院麻醉学教研室; |
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| 基金项目： | 国家自然科学基金(30571788) |
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| 摘 要： | 目的：探讨细胞周期末期促进复合物（APC）-Cdh1在中枢神经系统中的表达分布特点.方法：取健康成年雄性SD大鼠脑组织制作冰冻切片,免疫组化染色检测Cdh1的表达部位;免疫荧光双标检测Cdh1表达的细胞定位情况.取出生24h内乳鼠,原代培养神经元及神经干细胞,免疫组化检测NeuN进行神经元鉴定;Nestin染色进行神经干细胞初步鉴定;采用GFAP,MAP2免疫组化染色鉴定NSC分化特性.分别提取神经干细胞与神经元总RNA,采用实时定量PCR检测APC2个调节亚基Cdh1与CDC20的表达.结果：大鼠脑片免疫组化DAB染色显示Cdh1在海马、皮层均有大量表达,免疫荧光双标显示Cdh1表达主要定位于神经元中,星形胶质细胞表达较少.与神经干细胞相比,神经元中Cdh1 mRNA表达升高（1.00±0.05）vs（2.10±0.07）,P〈0.05],但CDC20 mRNA几乎没有表达（1.00±0.04,0.02±0.01,P〈0.05）.结论：APC-Cdh1在大鼠脑组织中有大量表达,主要定位于神经元,且神经元中Cdh1表达高于神经干细胞.
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| 关 键 词： | 细胞周期末期促进复合物 神经元 神经干细胞 |
Characteristics of APC-Cdh1 expression in central nervous system |
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| YAO Wen-Long,ZHANG Chuan-Han,QIAN Wei,QIU Jin,LIU Lu,ZHU Chang,GUI Ling-Li.Characteristics of APC-Cdh1 expression in central nervous system[J].Journal of the Fourth Military Medical University,2009(13):1161-1164. |
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| Authors: | YAO Wen-Long ZHANG Chuan-Han QIAN Wei QIU Jin LIU Lu ZHU Chang GUI Ling-Li |
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| Institution: | YAO Wen-Long,ZHANG Chuan-Han,QIAN Wei,QIU Jin,LIU Lu,ZHU Chang,GUI Ling-LiDepartment of Anesthesiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430030,China |
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| Abstract: | AIM:To investigate the characteristics of APC-Cdh1 expression in the central nervous system. METHODS: Fresh frozen sections were prepared from the brain tissue of health adult male SD rats and the expression of Cdh1 was detected by immunohistochemistry. The subtypes of Cdh1 positive cells were determined by double immunofluorescent labeling technique with Cdh1+NeuN or Cdh1+GFAP antibody. Primary neurons and neural stem cells from the hippocampus of postnatal 24 h rat pups were cultured. Neurons were identif... |
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| Keywords: | anaphase promoting complex neuron neural stem cells |
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