白藜芦醇对人肝癌细胞株SMMC-7721增殖和凋亡的影响

目的观察白藜芦醇对人肝癌细胞株SMMC-7721增殖和凋亡的影响，并初步探讨其机制。方法用MTT法检测白藜芦醇对SMMC-7721细胞增殖影响的量效与时效关系；应用流式细胞术（FCM）检测细胞凋亡率；比色法测定caspase-3酶活性。结果白藜芦醇（50、100、200μmol·L^-1）处理SMMC-7721细胞48h，呈浓度依赖性抑制SMMC-7721细胞增殖且诱导其凋亡；100μmol·L^-1白藜芦醇处理细胞24、48或72h显著抑制SMMC-7721细胞增殖并诱导其凋亡，且呈时间依赖性。白藜芦醇（50、100、200μmol·L^-1）处理SMMC-7721细胞48h，呈浓度依赖性增加SMMC-7721细胞caspase-3活性。结论白藜芦醇可抑制人肝癌细胞株洲C-7721的增殖，诱导细胞凋亡，其机制与增强细胞内caspase-3活性有关。

Effect of resveratrol in inducing growth inhibition and apoptosis of human hepatocellular carcinoma cells

Objective To explore the effect and mechanism of resveratrol on growth inhibition and apoptosis of human hepatocellular carcinoma cell line SMMC-7721. Methods The dose- and time-effect of resveratrol on the proliferation of SMMC-7721 cells were analyzed with MTT assay. The apoptosis was analyzed using flow cytometry with Annexin- V and PI staining, and caspase-3 activity was also measured with colorimetry. Results Exposed to resveratrol （50, 100 or 200 μmol. L^-1 ） for 48 h, the proliferation of SMMC-7721 cells was inhibited and the apoptosis was accelerated, both in a dose-dependent manner. Exposed to resveratrol （100 μmol.L^-1 ） for 24, 48 or 72 h, the proliferation of SMMC-7721 cells was inhibited and the apoptosis was accelerated, both in a dose-dependent manner. Treatment with resveratrol （50, 100 or 200 μmol.L^-1） for 48 h, the caspase-3 activity was significantly elevated in a time-dependent manner. Conclusion Resveratrol can induce significant growth inhibition and apoptosis of SMMC-7721 cells by mechanism related to easpase-3 pathway.