| RP-HPLC法同时测定丹七片中三七皂苷R_1和人参皂苷Rg_1的含量 |
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| 引用本文： | 蔡向阳,夏金华. RP-HPLC法同时测定丹七片中三七皂苷R_1和人参皂苷Rg_1的含量[J]. 中国药房, 2007, 18(36): 2837-2838 |
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| 作者姓名： | 蔡向阳 夏金华 |
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| 作者单位： | 1. 福建医科大学附属第二医院药剂科,泉州市,362000
2. 福建宁德市药品检验所,宁德市,352100 |
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| 摘 要： | 目的:建立以反相高效液相色谱法同时测定丹七片中三七皂苷R1和人参皂苷Rg1含量的方法。方法:色谱柱为KromasilC18(250mm×4.6mm,5μm),流动相为乙腈-水-磷酸(20.5∶79.5∶0.02),流速为1.0mL.min-1,柱温为室温,检测波长为203nm。结果:三七皂苷R1、人参皂苷Rg1的检测浓度分别在4.67~46.68(r=0.999 0)、4.62~115.50μg.mL-1(r=0.999 9)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为97.93%和97.98%,RSD分别为1.31%(n=6)和1.38%(n=6)。结论:本方法简便、快速、准确,可用于丹七片的质量控制。
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| 关 键 词： | 丹七片 三七皂苷R1 人参皂苷Rg1 反相高效液相色谱法 含量测定 |
| 文章编号： | 1001-0408(2007)36-2837-02 |
| 修稿时间： | 2007-06-27 |
Simultaneous Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Danqi Tablets by RP-HPLC |
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| CAI Xiangyang,XIA Jinhua. Simultaneous Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Danqi Tablets by RP-HPLC[J]. China Pharmacy, 2007, 18(36): 2837-2838 |
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| Authors: | CAI Xiangyang XIA Jinhua |
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| Abstract: | OBJECTIVE:To develop a RP-HPLC method for the simultaneous determination of Notoginsenoside R1 and Ginsenoside Rg1 in Danqi tablets. METHODS:The chromatographic separation was performed on a Kromasil C18(250mm×4.6mm,5μm) column with column temperature at room temperature. The mobile phase consisted of acetonitrile-water-H3PO4(20.5∶79.5∶0.02) at a flow rate of 1.0mL·min-1.The detection wavelength was set at 203nm. RESULTS:The linear range of Notoginsenoside R1 and Ginsenoside Rg1 were 4.67～46.68μg·mL-1 (r=0.999 0) and 4.62～115.50μg·mL-1 (r=0.999 9), respectively; with the average recover of Notoginsenoside R1 at 97.93% and that of Ginsenoside Rg1 at 97.98%, and RSD at 1.31%(n=6) and 1.38%(n=6), respectively. CONCLUSION:The method is simple, rapid and accurate, and suitable for the quality control of Danqi tablets. |
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| Keywords: | Danqi tablets Notoginsenoside R1 Ginsenoside Rg1 RP-HPLC Determination of content |
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