左金丸与反左金丸诱导人胃癌细胞SGC-7901凋亡的比较

目的比较左金丸（Zuojin Pill,ZP）和反左金丸（Retro-zuojin Pill,RZP）对人胃癌细胞（SGC-7901）生长的活性抑制和凋亡诱导作用。方法采用MTT比色法观ZP与RZP对SGC-7901的生长抑制情况；流式细胞仪PI单染亚二倍体峰分析细胞凋亡率；Hoechst 33258染色观察细胞形态学变化。结果0.1mg/ml ZP与RZP分别作用SGC-7901细胞后，均能抑制细胞活性，且与时间呈依赖关系。0.1、0.5和1.0mg/ml RZP作用SGC-7901细胞48h，细胞凋亡率为(5.52±.54)％、(6.91±2.31)%、(8.50±1.02)%，0.1、0.5和1.0mg/ml的ZP对细胞的凋亡率分别为(11.60±1.24）％、(21.80±0.28）%、(36.40±1.59）%，与对照组(1.69±1.91)%比较差异均有统计学意义（P＜0.01）。Hochest染色细胞出现核固缩状和颗粒状荧光等典型的凋亡学特征。结论左金丸和反左金丸对SGC-7901生长都有明显的抑制作用并可诱导SGC-7901的凋亡，同一浓度的ZP和RZP作用SGC-7901相同时间，ZP的作用效果更为明显。

Effect of Zuojin Pill and Retro-zuojin Pill on Apoptosis of SGC-7901 Cells

Objective To compare the cellular growth inhibition and apoptosis of human gastric carcinoma cells（SGC-7901)induced by Zuojin Pill（ZP）and Retro zuojin Pill（RZP). Methods The growth inhibition of the SGC 7901 treated by ZP or RZP were analyzed by MTT assay.The apoptosis rate of cell were studied by flow cytometry.Hoechst 33258 staining showed the cell morphology changes. Results After the cells treated by 0.1mg/ml ZP or RZP,the cell survival rate decreased in a time dependent manner.When the cells were treated with 0.1,0.5 and 1.0 mg/ml RZP for 48 h,the percentages of apoptosis were (5.52±0.54）％,(6.91±2.31）% and (8.50±1.02)% respectively.The same concentrations of ZP induced cell apoptosis at the percentage of (11.60±1.24）％,(21.80±0.28)% and(36.40±1.59)% respectively.The differences in comparison with the control group were statistically significant（P＜0.01). Hochest staining results showed cells nuclear condensation,granular fluorescence and other typical features of apoptosis. Conclusion ZP and RZP significantly inhibited the growth of SGC-7901 and induced the cell apoptosis where the effect of ZP was stronger than RZP under the same conditions.