银杏总内酯注射液对大、小鼠脑缺血的改善作用

目的:研究银杏总内酯注射液对大鼠实验性局部脑缺血的改善作用,并初步探讨其作用机制。方法:用三氯化铁(FeCl₃)造成大鼠大脑中动脉缺血模型(MCAO),观察对缺血大鼠行为状态、脑梗死面积及脑组织形态学的影响;应用肾上腺素-胶原蛋白诱导的小鼠体内血栓形成模型,观察该药整体抗血栓作用;应用二磷酸腺苷(ADP)诱导大鼠血小板聚集试验和葡聚糖-500造成的大鼠高血黏度试验,观察该药对血小板聚集和血液流变性的影响。结果:银杏总内酯注射液3.0 mg.kg^-1及以上剂量可改善MCAO大鼠的行为障碍,减少脑缺血区的梗死范围;6.0 mg.kg^-1及以上剂量可显著抑制小鼠体内血栓的形成,降低大鼠全血黏度;1.5 mg.kg^-1及以上剂量可明显降低ADP诱导的血小板聚集率。结论:银杏总内酯注射液对动物实验性脑缺血具有明显的改善作用,其作用机制可能与改善血凝状态、抑制血小板依赖性血栓的形成有关。

Effects of ginkgolides injection on experimental cerebral ischemia in mice and rats

Objective: To investigate the effects of ginkgolides injection on experimental cerebral ischemia and its related mechanism of action. Method: The middle cerebral artery occlusion(MACO) model was induced by the FeCl₃-occluding method to explore the protective effects of ginkgolides injection on the score of neurological deficits,the rate of cerebral infarction and the histomorphology of cerbral ischemia in rats.Thrombosis formation in vivo was induced by adrenaline-collagen in mice to explore the antithrombotic effect.Platelet aggregation was induced by ADP and hemorrheological parameters with hyper-viscosity by dextran T-500 were used to explore the effects of antiplatelet aggregation and decreasing viscosity of blood.Result: Ginkgolides injection could markedly decrease the infarct size and behavior deficits score,inhibit the thrombus formation in mice,decrease blood viscosity and ameliorate hemorrheological parameters in rat.Conclusion: Ginkgolides injection has the protective effects on focal cerebral ischemia,and its mechanism may be relative to its inhibition of platelet-dependent thrombosis and amelioration of hemarheological partments.