阻塞性睡眠呼吸暂停低通气综合征患者外周血淋巴细胞的DNA损伤及意义

目的探讨阻塞性睡眠呼吸暂停低通气综合征（obstructive sleep apnea hypopnea syndrome，OSAHS）患者外周血淋巴细胞DNA损伤及意义。方法选择在兰州大学第一附属医院就诊，经睡眠监测（PSG）确诊的30例男性0SAHS患者和22例男性健康志愿者，并用Epworth嗜睡量表（ESS）进行评估。用胞质阻滞法（cytokinesis blocked micronucleus，CBMN）检测外周血淋巴细胞DNA损伤情况。结果OSAHS组外周血微核发生频率（以下简称微核率）显著高于对照组．（38．60±11．85）％vs（12．40±5．47）‰（P〈0．01），且OSAHS组的微核率在轻、中、重各组闻差异有统计学意义，（28．46±8．42）‰vs（38．01±10．46）‰vs（42．43±11．72）‰（P〈0．05）。OSAHS组的微核率与睡眠呼吸低能气指数、夜间最低血氧饱和度、夜间平均血氧饱和度均有相关性（rs=0．517，P〈0．01；rs=0．549，P〈0．01；rs=-0．370，P〈0．05）。而OSAHS组染色体桥的发生频率也高于对照组，（7．65±6．30）‰vs（3．93±2．23）％（P〈0．05）。结论OSAHS患者外周血淋巴细胞存在较高的DNA损伤现象，这种损伤可能与间断性缺氧引起的氧化应激相关，并且可能进一步参与了心脑血管等靶器官损害的发病机制。

Significance of DNA damage in peripheral blood lymphocytes of patients with OSAHS

Objective To investigate the level of DNA damage in peripheral blood of patients with obstructive sleep apnea hypopnea syndrome(OSAHS).Methods Thirty male patients with OSAHS and 22 healthy male volunteers diagnosed by polysomnography(PSG) were chosen from the First Hospital of Lanzhou University,and were assessed by the Epworth Sleepiness Scale(ESS).The level of DNA damage was measured by the cytokinesis blocked micronucleus(CBMN).Results In the group of patients with OSAHS,the mean frequency of binucleate...