猕猴组织工程化周围神经的体外构建

目的:研究以猕猴骨髓基质干细胞作为种子细胞和以PLGA作为支架材料,体外构建猕猴组织工程化周围神经的方法.方法:采用密度梯度离心的方法分离培养猕猴骨髓基质干细胞,利用相差显微镜观察细胞生长情况,描绘其生长曲线;将4/0vicryl编织线在10倍手术显微镜下打散成PLGA细丝纤维,扫描电镜下观测其表面形态;将猕猴骨髓基质干细胞种植到经生物学修饰的PLGA纤维上共培养,倒置显微镜下观察细胞在支架材料上的生长情况.结果:采用密度梯度离心的方法可得到大量增殖能力强的猕猴骨髓基质干细胞;接种后的骨髓基质干细胞很快粘附于PLGA纤维上,生长、分裂、增殖良好,在PLGA纤维表面形成串珠样排列,部分细胞还形成连接在PLGA纤维间的链状或片状细胞桥.结论:猕猴骨髓基质干细胞与PLGA适合体外构建组织工程化周围神经.

The Consturction of Rhesus Tissue Engineered Peripheral Nerve in Vitro

Objective: To study the constructed method of Rhesus tissue engineered peripheral nerve(TEPN) using bone marrow stromal cell(BMSC)as seed cells and polylactic-acid-CO-polyglycolic-acid(PLGA) as scaffold in vitro.Method: Through density gradient centrifugation rhesus BMSC were got and observed under the phase contrast microscope.The growth curve of BMSC was described .The weave line of 4/0vicryl is broken up into PLGA fiber under operation microscope of 10 times,observe its surface under the scaning electron microscope;Plant Rhesus BMSC to PLGA fiber decorated by biology to train together,observe the growth under the phase contrast microscope.Result: Rhesus BMSC can be got in mass by density gradient centrifugation.;Rhesus BMSC can adhere to PLGA fiber quickly after plant and its growth,division,proliferration is good;Some cells arrange like rosary on PLGA fiber surface,some cells still form the catenate or flaky cell bridge joined among PLGA fiber.Conclusion: Rhesus BMSC and PLGA are suitable to construct Rhesus TEPN in vitro.