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| β 防御素 2 对大鼠肺部炎症细胞因子的影响 | |
| 引用本文： | 沈桢巍,方路,姜敏敏,刘显东,雷撼. β 防御素 2 对大鼠肺部炎症细胞因子的影响[J]. 成都医学院学报, 2013, 8(1): 17-20 |
| 作者姓名： | 沈桢巍 方路 姜敏敏 刘显东 雷撼 |
| 作者单位： | 沈桢巍 (同济大学附属东方医院重症监护室,上海,200120)；方路 (同济大学附属东方医院肾脏内科,上海,200120)；姜敏敏 (上海市杨浦区中心医院呼吸内科,上海,200090)；刘显东 (同济大学附属东方医院重症监护室,上海,200120)； 雷撼 (同济大学附属东方医院呼吸内科,上海,200120)； |
| 基金项目： | 国家自然科学基金面上项目（NO：30670932）;浦东新区卫生局面上项目（PW2005A-10）;浦东新区卫生局面上项目（PWRd2007-17） |
| 摘 要： | 目的 探讨 β 防御素 2 对大鼠肺部炎症反应的影响及其可能机制。方法 构建大鼠β 防御素 2（rBD2）的 cDNA 慢病毒载体和 RNA 干扰载体，双向调节 rBD2 在大鼠肺组织内的表达水平，ELISA 法检测肺组织中 IL-1α ，IL-1β ，IL-4，IL-10 的表达变化。Western blot检测肺组织中 rBD2 的表达，Real time PCR 法检测肺组织中 rBD2 mRNA 的表达。结果 成功构建 rBD2 cDNA 慢病毒和 RNA 干扰载体，并成功转染。大鼠肺部感染时，上调rBD2可降低IL-1α ，IL-1β等促炎细胞因子的水平，提高抗炎细胞因子IL-4，IL-10的含量（P-〈0．01）。结论rBD2可调节大鼠肺组织炎症相关细胞因子的水平，参与并抑制大鼠肺部炎症反应。 |
| 关 键 词： | 大鼠 β防御素2 细胞因子 |
The Impact of rBD2 on the Lung Inflammatory Cytokines of Rat |
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| Abstract: | Objective To study the impact of rat beta defensin-2（rBD2） on the pulmonary inflammation of rat and the possible mechanism. Methods To establish rat rBD2 cDNA lentiviral vector and vector for rBD2 siRNA. Both upregulation and downregulation of rBD2 expression were conducted to examine its effect on the pulmonary inflammation of rat. ELISA assay was conducted to evaluate the expression of cytokines,including IL-1α, IL-1β, IL-4 and IL-10. The expression of rBD2 in the lung was detected by Western blot; the expression of rBD2 mRNA was detected with Real-time PCR. Results Overexpression and RNAi lentiviral recombinant vectors of rBD2 were successfully constructed. Up regulation of rBD2 expression could decrease the level of IL-1α, IL-1β and increase the level of IL-4,IL-10（P（0.01）. Conclusion rBD2 could regulate the expression of cytokines and play a role as an anti-inflammatory role in the development of pulmonary inflammation in rat. |
| Keywords: | Rat β -defensin 2 Cytokine |
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