青龙衣多糖对S180小鼠红细胞Ca2+,Mg2+-ATP酶活性及[Ca2+]i的影响

目的考察青龙衣多糖对S180小鼠红细胞Ca2 ,Mg2 -ATP酶活性及[Ca2 ]i的影响。方法青龙衣多糖对S180小鼠ip给药7 d,比色法结合试剂盒测定S180小鼠红细胞膜Ca2 ,Mg2 -ATP酶的活性,采用激光共聚焦显微镜结合Fluo-3/AM荧光探针测定红细胞内[Ca2 ]i。结果青龙衣多糖能提高S180小鼠红细胞膜Ca2 ,Mg2 -ATP酶活性(P<0.05);降低S180小鼠红细胞[Ca2 ]i(P<0.05),并且青龙衣多糖的作用呈剂量依赖性,以高剂量组的作用最佳(P<0.01)。结论青龙衣多糖通过提高S180小鼠红细胞膜Ca2 ,Mg2 -ATP酶的活性,排除离子跨膜转运障碍,稳定其能量代谢和物质代谢,有利于维持细胞内Ca2 的正常浓度。

Effect of Qinglongyi polysaccharide on Ca2+,Mg2+-ATPase activity and[Ca2+]i in erythrocyte of S180 mice

Objective To study the effect of Qinglongyi polysaccharide on Ca2 ,Mg2 -ATPase acti-vity and [Ca~(2 )]_i in erythrocyte of S180 mice.Methods S180 Mice were ip administrated with Qinglongyi polysaccharide for 7 d.The Ca2 ,Mg2 -ATPase activity was observed by spectrophotometer with test kit and the [Ca~(2 )]_i in erythrocyte of S180 mice was measured by laser scanning cofocal microscope(LSCM) with Fluo-3/AM.Results It showed that Qinglongyi polysaccharide increased Ca2 ,Mg2 -ATPase acti-vity(P<0.05),decreased the [Ca2 ]i in erythrocyte of S180 mice(P<0.05) in a dose dependent manner.The effect in higher dosage was the best(P<0.01).Conclusion Qinglongyi polysaccharide could in-crease the Ca2 ,Mg2 -ATPase activity in erythrocyte of S180 mice,resolve the obstacle of ion trans-portion,stabilize the metabolism of energy and materials,and maintain the normal concentration of Ca2 .