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| 小鼠脑Ca~(2+)/CaM依赖性蛋白激酶Ⅱ的鉴定及苄普地尔对其酶活力的抑制作用 | |
| 引用本文： | 唐放鸣,张光毅,赵昇皓,彭振云. 小鼠脑Ca~(2+)/CaM依赖性蛋白激酶Ⅱ的鉴定及苄普地尔对其酶活力的抑制作用[J]. 徐州医学院学报, 1990, 0(2) |
| 作者姓名： | 唐放鸣 张光毅 赵昇皓 彭振云 |
| 作者单位： | 徐州医学院生物化学教研室(唐放鸣，张光毅，赵昇皓)，徐州医学院生物化学教研室(彭振云) |
| 摘 要： | 用DE_(52)、磷酸纤维素和Sephaery S—300柱层析从小白鼠脑中提纯了Ca~(2+)/CaM依赖性蛋白激酶Ⅱ并进行了鉴定。凝胶过滤法测定全酶分子量为55万,在SDS-PAGE中显示出两条区带,其亚基分子量分别为5万和5.7万。该酶活力依赖于Ca~(2+)和CaM,AC_(50)分别为28μmol/L和2.2μmol/L。苄普地尔对酶有抑制作用,IC_(50)约为250μmol/L。 |
| 关 键 词： | Ca~(2+)/caM依赖性蛋白激酶Ⅱ 钙离子 钙调素 苄普地尔 小鼠 |
CHARACTERIZATION OF Ca~2+/CaM DEPENDENT PK II FROM MOUSE BRAIN AND INHIBITION ON Ca~2+/CaM DEPENDENT PK II ACTIVITY BY BEPRIDIL |
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| Tang Fangming. CHARACTERIZATION OF Ca~2+/CaM DEPENDENT PK II FROM MOUSE BRAIN AND INHIBITION ON Ca~2+/CaM DEPENDENT PK II ACTIVITY BY BEPRIDIL[J]. Acta Academiae Medicinae Xuzhou, 1990, 0(2) | |
| Authors: | Tang Fangming |
| Abstract: | Ca2+/CaM dependent protein kinase II was separated from mouse brain by DEAE cellulose and phosphate cellulose column chromatography further purified and characterized by Sephacryl S-300 column chromatography and SDS-polyacrylamide gel electrophoresis. The molecular weight of purified enzyme was estimated by Sephacryl S-300 to be 550000 . Two bands were shown on SDS-PAGE, with relative mobilities corresponding to two subunits molecular weight about 50000 and 57000.The activity of the enzyme was dependent on Ca2+ and CaM, (AC50 value 28 and 2,2umol/L, respectively) and was inhibited by Bepridil, with IC50 value about 250umol/L. |
| Keywords: | Ca2+/CaM dependent protein kinase calcium calmodulin bepridil mouse |
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