VEGFR-3、LYVE-1在胃癌中的表达研究

目的比较VEGFR-3、LYVE-1在胃癌组织中的表达,选择较为可靠的淋巴管内皮标志物。方法利用免疫组织化学方法检测CD34、VEGFR-3和LYVE-1在53例胃癌组织及正常胃粘膜组织中的表达,并进行微淋巴管密度（lymphatic vessel density,MLD）及微血管密度（microvessel density,MVD）计数;与公认的血管内皮标志物CD34作比较,评价VEGFR-3和LYVE-1两种淋巴管内皮标志物的表达情况。结果 CD34和VEGFR-3阳性脉管密度具有相关性（r=0.387,P=0.003）;而LYVE-1与CD34阳性管数不相关（r=-0.181,P=0.232）,表明VEGFR-3阳性管多数是血管,LY-VE-1阳性管多数是淋巴管而极少数是血管,提示LYVE-1是较为特异的淋巴管内皮标志物。结论 LYVE-1是较为特异的淋巴管内皮标志物,肿瘤瘤周淋巴管生成可能促进了淋巴结转移的发生。

Expression and Significance of VEGFR-3 and LYVE-1 in gastric carcinoma

Objective To select the most specific lymphatic endothelial marker through expression between LYVE-1 and VEGFR-3 in gastric cancer.MethodsThe expression of CD34,VEGFR-3,and LYVE-1 was quantified in 53 sections and the normal tissues using immunohistochemistry.Micro-lymphatic vessel density（MLD） and microvessel density（MVD） were measured.The specificity of lymphatic vessel endothelium markers was compared between LYVE-1 and VEGFR-3.ResultsA positive correlation was found between the number of CD34 and VEGFR-3 positive vessels（r=0.387,P=0.003）,while a negative correlation was found between CD34 and LYVE-1（r=-0.181,P=0.232）,indicating that most VEGFR-3 vessels and few LYVE-1 positive vessels were blood vessels,while most LYVE-1 positive vessels were lymphatic vessels.Thus LYVE-1 showed to be the more specific marker of lymphatic vessel endothelium.ConclusionLYVE-1 is a more specific lymphatic endothelial marker.It is also suggested that peritumoral lymphangiogenesis might promote lymph node metastasis.