| 老年大鼠脑细胞内钙的变化 |
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| 引用本文： | 曹云鹏,李智,丛华,聂华,魏敏杰.老年大鼠脑细胞内钙的变化[J].中国医科大学学报,2005,34(5):418-419. |
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| 作者姓名： | 曹云鹏 李智 丛华 聂华 魏敏杰 |
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| 作者单位： | [1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]基础医学院药理学系,辽宁沈阳110001 |
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| 摘 要： | 目的:观察老年大鼠脑细胞内钙浓度(Ca2 ]i)的变化.方法:以Fura/AM为荧光指示剂,采用荧光分光光度计测定急性分离大鼠脑Ca2 ]i.结果:静息状态下老年大鼠脑Ca2 ]i及对高钾除极化的反应均明显低于青年对照组(P＜0.01),但其增强作用在老年大鼠与青年大鼠间无明显差异(P＞0.05).结论:老年大鼠静息脑Ca2 ]i降低,但在相同情况下老年大鼠脑细胞内钙超载更加明显,从而较青年大鼠更易发生脑细胞的损害.
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| 关 键 词： | 老年大鼠 脑细胞 细胞内钙 |
| 文章编号： | 0258-4646(2005)05-0418-02 |
| 收稿时间： | 2004-11-28 |
| 修稿时间： | 2004年11月28 |
Change of intracellular calcium in brain cells of aged rats |
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| CAO Yun-peng, LI Zhi, CONG Hua, NIE Hua, WEI Min-jie.Change of intracellular calcium in brain cells of aged rats[J].Journal of China Medical University,2005,34(5):418-419. |
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| Authors: | CAO Yun-peng LI Zhi CONG Hua NIE Hua WEI Min-jie |
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| Institution: | 1. Department of Neurology, The First Affiliated Hospital ,China Medical University, Shenyang 110001 ,China; 2. Department of Pharmacology, College of Basic Medical Sciences |
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| Abstract: | Objective: To observe the change of intracellular calcium(Ca~(2+)]i) in brain cells of aged rats.Methods:Fluorescence spectrophotometer with fura-2 as a calcium fluorant was used to measure Ca~(2+)]i in acutely dissociated brain cells of aged rats.Results:In the presence of 1 mmol/L of extracellular Ca~(2+),resting Ca~(2+)]i and the response of Ca~(2+)]i to rise in KCl in brain cells of aged rats were all significantly reduced than those of young rats.But there was no significant difference in the increase of Ca~(2+)]i induced by different concentrations of KCl Ca~(2+)]i between the aged and the young rats(P>0.05).Conclusion:Resting Ca~(2+)]i in brain cells of aged rats is reduced.The overoad of Ca~(2+)]i in brain cells of aged rats is more significantly than that in young rats under same circumstances,so the brain cells of aged rats are more lirely to be damaged. |
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| Keywords: | aged rots brain cell intracellular calcium |
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