白杨素对人胃癌SGC-7901细胞的体内抑制作用

目的观察白杨素（Chrysin,ChR）对人胃癌SGC-7901细胞的体内抑制作用。方法建立人胃癌SGC-7901细胞株Balb/c-nu裸鼠异种移植瘤模型,共24只,随机分成4组,每组6只,即对照组、白杨素30mg/kg治疗组、白杨素60 mg/kg治疗组、白杨素120 mg/kg治疗组。观察不同剂量白杨素对荷瘤裸鼠肿瘤生长的抑制作用。通过流式细胞术分析各组肿瘤细胞的凋亡率和增殖百分比。结果各治疗组肿瘤重量明显小于对照组（P〈0.01）,对照组及30 mg/kg、60 mg/kg、120 mg/kg白杨素治疗组对移植瘤的抑瘤率分别是0%、23.80%、40.36%、61.19%。各组凋亡率分别是（7.8±3.7）%、（15.2±2.2）%、（22.0±3.1）%、（53.0±9.2）%,白杨素60mg/kg和120 mg/kg治疗组抑制作用均明显大于对照组（P〈0.01）。结论白杨素对人胃癌SGC-7901细胞具有体内抑制作用,其机理可能与诱导肿瘤细胞凋亡相关。

Inhibitory Effect of Chrysin on the Gastric Cancer SGC-7901 Cells in Vivo

Objective To study the ihibitiory effect of chrysin on the gastric cancer cells（ SGC -7901 ） in vivo and to explore the mechanisms of the effect. Methods 24 molels of human gastric carcinoma xenograft in Balb/c - nude mouse were established to be divided into four groups randomly （6 in each group） ： control group, 30 mg/kg chrysin group, 60 mg/kg chrysin group, 120 mg/kg chrysin group. The inhibitory effect on tumors in each group was studied. The apoptosis rate and s - phase fraction were analyzed by Flow Cytometry. Results The average weight of tumors in treated groups were significantly less than which in the control group Ⅰ P 〈0. 01 ） ,the inhibitory rate was 0% ,23.80% ,40.36% ,61.19% respectively. The apoptosis rate was （ 7.8 ± 3.7 ） %, （ 15.2 ± 2.2 ） %, （ 22.0 ± 3.1 ） %, （ 53.0 ± 9.2 ） % respectively, the apoptosis rate of group 60 mg/kg and 120 mg/kg were significantly lower compared with control group （ P 〈 0.01 ）. Conclusion Chrysin inhibits the growth of SGC -7901 grastic cancer in vivo. The mechanism for the effect might be related to the apoptosis inducement.