|
|||||
|
|
| FAME24/20中两步法ELISA试剂工作表的优化设计 | |
| 引用本文： | 李文,丁显平,王乃红,杨群身. FAME24/20中两步法ELISA试剂工作表的优化设计[J]. 现代预防医学, 2007, 34(11): 2060-2062 |
| 作者姓名： | 李文 丁显平 王乃红 杨群身 |
| 作者单位： | 1. 成都市血液中心,成都,610041;四川大学生命科学学院遗传医学研究所/生物资源与生态环境教育部重点实验室 2. 四川大学生命科学学院遗传医学研究所/生物资源与生态环境教育部重点实验室 3. 成都市血液中心,成都,610041 |
| 摘 要： | [目的]为了缩短FAME24／20全自动酶联免疫分析仪的工作时间，提高工作效率。[方法]在FAME用户软件中编制好实验步骤，指定洗液的洗板单元和试剂的分配单元，改进酶标板进板方式和顺序，对ELISA两步法试剂的工作表进行优化。[结果]编制出2个能在53min内完成15个酶标板进板的工作表。[结论]优化设计两步法ELISA试剂的工作表，可以充分利用FAME24／20的仪器资源，并有效提高其使用效率。 |
| 关 键 词： | FAME全自动酶联免疫分析仪 两步法ELISA 工作表 优化设计 |
| 文章编号： | 1003-8507（2007）11-2060-03 |
| 收稿时间： | 2007-01-26 |
| 修稿时间： | 2007-01-26 |
THE WORK LIST OPTIMIZATION OF TWO-STEP ELISA REAGENT IN FAME 24/20 |
|
| LI Wen, DING Xian-ping, WANG Nai-hong,et al.. THE WORK LIST OPTIMIZATION OF TWO-STEP ELISA REAGENT IN FAME 24/20[J]. Modern Preventive Medicine, 2007, 34(11): 2060-2062 | |
| Authors: | LI Wen DING Xian-ping WANG Nai-hong et al. |
| Affiliation: | LI Wen, DING Xian-ping, WANG Nai-hong, et al. |
| Abstract: | [Objective]To reduce the work time and increase the work efficiency of Fully Automated Microplate ELISA Analyzer 24/20(FAME 24/20).[Methods]FAME user software was used to define the test processing steps,the washer module of wash solution was specified,the dispenser module of reagent was defined and the loading mode and order of ELISA microplate was improved. The work list of two-step ELISA reagent was optimized.[Results]We scheduled two work lists which could load 15 ELISA microplate in 53 min.[Conclusion]By optimizing the work list of two-step ELISA reagent,the resource of FAME 24/20 is fully used and the using effiency of it is also improved. |
| Keywords: | Fully Automated Microplate ELISA Analyzer Two-step ELISA Work list Optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |