急性脊髓损伤后胎鼠脊髓的脊髓内移植及其影响因素

本文利用显微外科技术建立了大鼠急性脊髓损伤后胎鼠脊髓的移植方法,并用形态学技术研究了胎龄及其他因素对移植物的影响。结果表明,在E_(14)～E_(20)胎龄中,以取自E_(14)的移植物存活率最高,为70.83％。E_(16)以后,无一例移植物存活。附于移植物的结缔组织严重阻碍移植物的存活与生长。脊髓损伤及继发的病理反应也对移植物的存活与生长带来不良影响。我们认为,胚胎神经组织移植时,除注意技术操作和改善脊髓损伤外,事先确定移植物的分化程度至关重要。

A sduty on transplantation of embryonic spinal cord and favorable factors for its acceptance after acute spinal cord injury in rats

Transplantation of embryonic spinal cord was performed with microsurgical technique in rats after they were inflicted with acute spinal cord injury.Then the favorable factors affecting the acceptance of the transplanted cord were investigated with morphological technique.It was found that among the gestation ages from EH to E20,the spinal cord taken from the rats with the gestation age of E14 showed the highest survival rate of 70.83%.None of the transplants taken from the rats with the gestation age older than E14 survived.The connective tissue attached to transplants severely interfered with their acceptance and growth.The severity of the pathological manifestations of the injured spinal cord exerted unfavorable influence on the survival and growth of the transplants too.It is believed that in order to assure the success of the transplantation,besides the attention on the technical performance of the surgery,it is essential to determine the differentiation state of the embryonic spinal cord to be transplanted.