昆藻调脂胶囊对实验性高脂血症性脂肪肝大鼠肝细胞增殖的影响

目的:探讨昆藻调脂胶囊治疗高脂血症性脂肪肝的作用机制。方法:取59只大鼠随机分为正常对照组(n=10)、模型组(n=11)、阳性对照组(n=10)及昆藻调脂胶囊高、中、低剂量组(n=9、9、10),除正常对照组外,其余各组采用高脂饲料及30%乙醇方法复制脂肪肝模型,同时给予相应溶剂或药物,10wk后运用流式细胞术观察各组大鼠肝细胞DNA的S期细胞比例(SPF)及增殖指数(PI)。结果:与正常对照组比较,造模后大鼠PI、SPF值明显升高(P<0.05);昆藻调脂胶囊能降低造模后大鼠PI、SPF值(P<0.05),尤其对♀大鼠的作用更为显著(P<0.01)。结论:昆藻调脂胶囊通过抑制高血脂大鼠特别是♀大鼠肝细胞的增殖而发挥抗肝纤维化作用。

Effects of Kunzao Tiaozhi Capsule on Cell Proliferation in Experimental Rats with Fatty Liver and Hyperlipidemia

OBJECTIVE: To study the action mechanism of kunzao tiaozhi capsule in the treatment of fatty liver with hyperlipidemia.METHODS: A total of 59 rats were randomly divided into normal control group(n=10),model group(n=11),positive control group(n=10) and kunzao tiaozhi capsule group(at high,medium and low doses,n=9,9,10).Rat model with fatty livers was established in all the groups except the normal control group by feeding high-fat forage and 30% alcohol,and the corresponding solvents or medicine were given during the modeling,10 weeks later,the S-phase cell percentages(SPF) of the liver cells in rats and proliferation index(PI) were determined by flow cytometry methods.RESULTS: As compared with the normal control group,PI and SPF values in the modeled rats increased significantly(P<0.05),while which were decreased in kunzao tiaozhi capsule group after modeling(P<0.05),especially in the female rats(P<0.01).CONCLUSION: The anti-hepatic fibrosis effect of kunzao tiaozhi capsule can be achieved by inhibiting the cell proliferation in rats with hyperlipidemia(especially the female rats) .