N-硝基-L-精氨酸甲基酯对阿霉素致大鼠心肌损伤的影响

目的 研究一氧化氮合酶抑制剂N-硝基-L-广精氨酸甲基酯（L-NAME）对阿霉素（ADM）致大鼠心肌损伤的保护作用。方法 85只雄性Wistar大鼠，随机分为ADM组、ADM＋L-NAME组及对照组。实验第30天，光镜下观察心肌形态结构病理改变；测定血清及心肌组织中的脂质过氧化物（MDA）和超氧化物歧化酶（SOD）活性；测定心肌组织中的一氧化氮（NO）及诱导型一氧化氮合酶（iNOS）的活性。结果 光镜下ADM＋L-NAME组大鼠心肌病理损害程度较ADM组轻，ADM＋L-NAME和ADM两组心肌坏死灶的检出率分别为16.7％和43.3％。二者比较差异显著意义（P〈0.05）。与ADM组比较，ADM＋L-NAME组血清、心肌组织中的MDA显著降低，而SOD活性显著增加；与对照组比较，ADM组心肌中NO和iNOS的活性均显著升高。结论 L-NAME对ADM致大鼠心肌损伤有保护作用，其机理可能与抑制iNOS活性使心肌组织NO减少有关。

Effect of NG-nitro-L-argininemethyl-ester on rat myocardial injury induced by adriamycin

Objective To study the effect of NG-nitro-L-argininemethyl-ester(L-NAME)on rat myocardial injury induced by adriamycin(ADM).Methods Eighty-five male Wistar rats were randomly divided into three groups:ADM group,ADM L-NAME group and control group.MDA,SOD,nitric oxide(NO)and inducible nitric oxide synthase(iNOS)in myocardium and serum were detected and the pathological study was performed.Results The extent of myocardial injury in ADM L-NAME group(16.7%)was significantly lower than that of ADM group(43.3%)with P<0.05.Compared with ADM group,the contents of MDA in serium and myocardium of ADM L-NAME group decreased significantly while the activity of SOD increased significantly.Compared with control group,the contents of nitric oxide and iNOS activity of myocardium in ADM group increased significantly.Conclusion L-NAME has protective effect on rat myocardial injury induced by ADM.The mechanism of the protective effect might related to the suppression of the iNOS activity which decreases the nitric oxide in myocardium.