肺炎衣原体(Cpn)诱发肺癌大鼠模型

 目的 初步探索肺炎衣原体(Chlamydia pneumoniae,Cpn)诱发大鼠肺癌模型的建立。方法 应用多次气管内注入Cpn菌液(TW 183)的方法试制肺癌大鼠模型,微量免疫荧光(microimmunofluorescence,MIF)法检测大鼠血清中Cpn特异性抗体,PCR检测肺癌组织中Cpn DNA,酶联免疫法检测肺癌组织中Cpn特异性抗原。结果 单独Cpn感染组Wistar鼠的Cpn感染率为72.9%(35/48),Cpn感染联合苯并芘(benzopyrene,Bp)组的Cpn感染率为76.7%(33/43),两组的肺癌发生率分别为25.0%(12/48)和44.2%(19/43),与正常对照组相比,P值分别为0.001和0.000。结论 Cpn感染诱发大鼠肺癌模型的建立是成功的,有助于肺癌的防治及其发病机制研究。

Preliminary study on lung cancer rat model produced by Chlamydia pneumoniae(Cpn)

Objective Preliminary study on rat model of lung cancer produced by Chlamydia pneumoniae(Cpn). Methods The lung cancer animal model was developed by intratracheal repeated injecting Cpn(TW-183) into lung of rat.The Cpn-antibodies(Cpn-IgA,-IgG and-IgM) of rat serum were measured by microimmunofluorescence(MIF) method.The Cpn-DNA or Cpn-Ag of rat lung cancer were detected by PCR method or ELISA. Results The prevalence of Cpn infection was 72.9%(35/48) in Cpn group,and 76.7%(33/43) in Cpn plus benzopyrene(Bp) group.The incidences of lung carcinomas of the two groups were 25.0%(12/48) and 44.2%(19/43).Compared with normal controls,P value were 0.001 and 0.000,respectively. Conclusions A rat model of lung carcinoma produced by Cpn was successfully prepared for further study in experiment on treatment,prevention and mechanism.