Preparation of dissociated Zebrafish spinal neuron cultures |
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Authors: | Andersen Søren S L |
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Institution: | (1) Department of Molecular and Cellular Biology, University of California at Berkeley, 142 Life Sciences Addition #3200, Berkeley, CA 94720-3200, USA |
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Abstract: | The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance. |
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Keywords: | growth guidance in vitro neuron Xenopus Zebrafish |
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