| 金银花中绿原酸提取工艺的比较和优化研究 |
| |
| 引用本文： | 刘祥兰,刘重芳,张英,陶建生.金银花中绿原酸提取工艺的比较和优化研究[J].中成药,2000,22(6):402-404. |
| |
| 作者姓名： | 刘祥兰 刘重芳 张英 陶建生 |
| |
| 作者单位： | 1. 上海中医药大学中药学院,上海,200032
2. 上海市市北医院,上海,200435 |
| |
| 基金项目： | 上海市教委科技发展基金项目,编号:97G-2 |
| |
| 摘 要： | 目的：优选金银花中绿原酸的提取工艺。方法：比较多种提取工艺，如传统水煎法，乙醇回流法，动态温浸法，超声波法，渗漉法等，正交设计筛选绿原酸的提取工艺，采用高效液相色谱法测定该成分含量结果：最佳提取工艺是乙醇回流。影响提取的主次因素为Ａ〉Ｃ〉Ｂ。最侍者条件是加１０倍量７５％乙醇，回流提取０．５ｈ。绿原酸含提取率均较高。结论：优选得到的工艺简单、稳定、可行
|
| 关 键 词： | 金银花 绿原酸 正交设计 提取工艺 中药 |
| 文章编号： | 1001-1528(2000)06-0402-03 |
| 修稿时间： | 1999年11月10 |
Study on Different Extraction Process of Chlorogenic Acid in Flos Lonicerae and Optimum |
| |
| LIU Xiang-lan,LIU Chong-fang,ZHANG Ying,TAO Jian-sheng.Study on Different Extraction Process of Chlorogenic Acid in Flos Lonicerae and Optimum[J].Chinese Traditional Patent Medicine,2000,22(6):402-404. |
| |
| Authors: | LIU Xiang-lan LIU Chong-fang ZHANG Ying TAO Jian-sheng |
| |
| Abstract: | Objective: To optimize the extraction process of chlorogenic acid in Flos Lonicerae. Methods: Several extraction processes were used respectively, such as traditional decocting method, the refluxing method, the ultrasonic method etc. The optimum extraction was investigated by the orthogonal design and chlorogenic acid determined by HPLC. Results: The optimum extraction is refluxing method. The factors influencing this extraction efficiency were as follows: A>C>B. (A: Consistency of ethanol, C: Times of reflux. B: Amount of ethanol) The optimum extraction conditions were: 10 fold of 75% ethanol, refluxing 0.5 h. The content or chlorogenic acid and extraction rate were fairly high. Conclusion: This optimized process is simple, stable and efficient. |
| |
| Keywords: | Flos Lonicerae Chlorogenic acid Orthogonal design Extraction process |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
