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Epigallocatechin-3-gallate induces apoptosis and cell cycle arrest in HTLV-1-positive and -negative leukemia cells |
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| Authors: | S. Harakeh K. Abu-El-Ardat M. Diab-Assaf A. Niedzwiecki M. El-Sabban M. Rath |
| Affiliation: | (1) Biology Department, American University of Beirut, Beirut, 11-0236, Lebanon;(2) Dr. Rath Research Insitute, Santa Clara, CA, USA;(3) Human Morphology Department, American University of Beirut, Beirut, 11-0236, Lebanon |
| Abstract: | The objective of this study is to evaluate the efficacy of epigallocatechin gallate against ATL cells. The anti-proliferative and pro-apoptotic effects of EGCG were evaluated in HTLV-1-positive and -negative cells. EGCG exhibited a marked decrease in proliferation of ATL cells at 96 h of treatment. The results indicated that TGF-α was down-regulated whereas levels of TGF-β2 increased. Cell cycle distribution analysis revealed an increase in cells in the pre-G1 phase which was confirmed by ELISA. The results on proteins showed an up-regulation of p53, Bax and p21 protein levels while the levels of Bcl-2α were down-regulated. |
| Keywords: | Apoptosis Human T-cell Lymphotrophic Virus-1 (HTLV-1) Epigallocatechin-3-gallate (EGCG) p53 p21 Transforming Growth Factor (TGF) |
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