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HPLC测定骨松宝颗粒中淫羊藿苷的含量
引用本文:张志锋,刘忠荣,及元乔,罗培.HPLC测定骨松宝颗粒中淫羊藿苷的含量[J].华西药学杂志,2005,20(4):351-352.
作者姓名:张志锋  刘忠荣  及元乔  罗培
作者单位:成都地奥集团药物研究所,四川,成都,610041;四川大学华西药学院,四川,成都,610041
摘    要:目的测定骨松宝颗粒中淫羊藿苷的含量。方法采用高效液相色谱法,色谱柱为C18柱(4.6mm×250mm,5μm),流动相为乙腈-水(26∶74),检测波长270nm。结果淫羊藿苷的线性范围为0.0331~0.3310mg.ml-1,回归方程为:Y=1.857×103X 11.18(r=0.9999)。结论所用方法简便、快速、准确、专属性好。

关 键 词:骨松宝颗粒  HPLC  淫羊藿苷
文章编号:1006-0103(2005)04-0351-02
收稿时间:2004-12
修稿时间:2004年12月1日

Determination of icariin content in Gusongbao granule by HPLC
ZHANG Zhi-feng,LIU Zhong-rong,JI Yuan-qiao,LUO Pei.Determination of icariin content in Gusongbao granule by HPLC[J].West China Journal of Pharmaceutical Sciences,2005,20(4):351-352.
Authors:ZHANG Zhi-feng  LIU Zhong-rong  JI Yuan-qiao  LUO Pei
Institution:ZHANG Zhi-feng~1,LIU Zhong-rong~1,JI Yuan-qiao~1,LUO Pei~2
Abstract:OBJECTIVE To determine icariin content in Gusongbao granule. METHODS HPLC method was performed on a C_(18) column.The mobile phase consisted of acetonitrile-water(2674) and the UV detective wavelength was set at 270 nm. RESULTS The calibration curve of icariin was linear within 0.0331-0.331 mgml~(-1), Y=1.85710~3X 11.180(r=0.9999).CONCLUSION This method is simple,rapid and accurate. It is suitable for the determination of icariin content.
Keywords:Gusongbao granule  HPLC  Icariin
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