凋亡抑制蛋白Livin在食管癌表达的实验研究

目的 探讨食管癌发生、发展过程中凋亡抑制蛋白Livin的表达及其与食管癌临床病理特征的关系.方法 采用实时定量PCR法及蛋白免疫印迹法检测Livin在正常食管黏膜(10例)、不典型增生(10例)、原位癌(10例)及浸润癌(10例)组织中mRNA及蛋白的表达强度.结果 Livin mRNA在正常食管黏膜、不典型增生、原位癌及浸润癌组织中呈渐进性高表达[分别为1.00±0.00、2.26±0.79、7.24±1.06、12.21±2.47],原位癌、浸润癌与正常食管黏膜组织比较,差异有统计学意义(P均<0.05).结论 Livin与食管癌的发生、发展密切相关,有望成为食管癌诊断和基因治疗的新靶点.

Detection of apoptotic inhibitor protein Livin in different esophageal carcinoma tissue

Objective To detect the expression of apoptotic inhibitor protein Livin in different esophageal epithelial lesions and to analyze the relation between the expression of Livin with pathologic characteristics. Methods The expressions of Livin mRNA and Livin protein were detected by real- time fluorescence quantitative PCR and western blot in 40 patients with different esophageal epithelial lesions including normal, atypical hyperplasia, carcino-ma in stiu and invasive carcinoma. Results The expressions of Livin mRNA were progressively increased from nor-mal to invasive carcinoma( 1.00 ± 0. 00,2.26 ± 0.79,7.24 ± 1.06,12.21 ± 2.47 ). There was statistical signifi-cance in Livin mRNA expression among carcinoma in stiu and invasive carcinoma and normal tissues ( P < 0.05 ). Conclusion The expression of Livin is significantly related to the progression of esophageal cancer,which may be a new target for diagnosis and gene treatment of esophageal carcinoma.