HPLC法测定黄龙咳喘胶囊中淫羊藿苷的含量 |
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引用本文: | 常润,夏传涛,方宇.HPLC法测定黄龙咳喘胶囊中淫羊藿苷的含量[J].西北药学杂志,2016(5):469-471. |
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作者姓名: | 常润 夏传涛 方宇 |
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作者单位: | 1. 西安交通大学药学院,西安 710061; 陕西东科制药有限责任公司,西安 710043;2. 陕西东科制药有限责任公司,西安,710043;3. 西安交通大学药学院,西安,710061 |
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摘 要: | 目的建立黄龙咳喘胶囊中淫羊藿苷的HPLC含量测定方法。方法采用HPLC法。色谱柱:十八烷基硅烷键合硅胶为填充剂(TIANHE Kromasil C_(18),150mm×4.6mm,5μm)色谱柱;流动相:乙腈-0.075mol·L~(-1)磷酸溶液(用三乙胺调pH值约为4.5)(25∶75);流速:1mL·min~(-1);柱温:30℃;检测波长:270nm。结果淫羊藿苷在0.008 1~0.081 0mg·mL~(-1)范围内呈良好的线性关系,平均回收率为98.7%,RSD值为1.62%(n=6)。结论该方法简便,结果准确可靠,可作为该胶囊的质量控制方法。
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关 键 词: | 黄龙咳喘胶囊 淫羊藿苷 高效液相色谱法 含量测定 |
Determination of icariin in Huanglongkechuan Capsules by HPLC |
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Abstract: | Objective To determine icariin in Huanglongkechuan Capsules by HPLC .Methods HPLC column:TIANHE Kromasil C18 (150 mm × 4 .6 mm ,5μm);mobile phase:acetonitrile‐0 .075 mol · L -1 phosphoric acid solution (adjusted pH of about 4 .5 with triethylamine)(25∶75);flow rate:1 mL · min-1 ;column temperature:30 ℃ ;detection wavelength :270 nm .Results Icariin with‐in 0 .008 1‐0 .081 0 mg · mL -1 showed a good linear relationship .The average recovery was 98 .7% ,with RSD 1 .62% (n=6) . Conclusion This method is simple ,accurate and reliable ,and can be used for the quality control of Huanglongkechuan Capsules . |
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Keywords: | Huanglongkechuan Capsules icariin HPLC determination |
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