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| 宫颈癌中抑癌基因FHIT表达与HPV16基因状态 | |
| 引用本文： | 徐又先,袁林,濮德敏,马丁. 宫颈癌中抑癌基因FHIT表达与HPV16基因状态[J]. 现代妇产科进展, 2016, 0(3): 182-186. DOI: 10.13283/j.cnki.xdfckjz.2016.03.030 |
| 作者姓名： | 徐又先 袁林 濮德敏 马丁 |
| 作者单位： | 1. 广西柳州市人民医院妇产科,柳州,545006;2. 华中科技大学同济医学院附属同济医院妇产科,武汉,430030 |
| 基金项目： | 国家自然科学基金资助项目(81160446)，柳州科技攻关资助项目(2013J030403)，柳州市培养新世纪学术和技术带头人专项资金资助项目(柳人社发(2015)141号) |
| 摘 要： | 目的:探讨抑癌基因FHIT表达及人乳头瘤病毒16(HPV16)的基因型整合状态在宫颈癌发生发展中的作用及相关性。方法:选取柳州市人民医院2013年6月至2014年12月收治的42例宫颈癌、55例宫颈上皮内瘤样病变(CIN)和20例宫颈正常组织的患者,免疫组化法检测宫颈组织中FHIT蛋白表达;多重PCR法检测HPV16 E2/E7表达。结果:FHIT蛋白的总阳性表达率为57.26%(67/117),正常宫颈组织、CINⅠ、CINⅡ、CINⅢ和宫颈癌中FHIT蛋白阳性率分别为85.00%、80.00%、75.00%、60.00%和26.19%。随着宫颈病变加重,FHIT蛋白阳性表达率下降,组间差异有统计学意义(χ~2=7.335;P=0.003)。117例单纯HPV16阳性标本HPV16总整合率为81.20%,正常宫颈组织、CINⅠ、CINⅡ、CINⅢ和宫颈癌中整合率分别为60.00%、66.67%、75.00%、95.00%和92.86%;随病变加重,整合率增强,组间差异有统计学意义(χ~2=5.713,P=0.003);FHIT蛋白阳性表达在不同HPV16整合时不同,差异有统计学意义(χ~2=11.989,P=0.000)。结论:HPV16基因整合可能通过诱导FHIT基因低表达从而促使宫颈癌发生发展。 |
| 关 键 词： | 人乳头状瘤病毒 脆性组氨酸三联体 整合 宫颈上皮内瘤样病变 宫颈癌 |
To explore the expression of FHIT and its relationship with the integration of HPV16 in cervical cancer |
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| Abstract: | Objective:To explore the expression of fragile histidine triad ( FHIT ) and its relationship with the integration of human papilloma viral 16 ( HPV16 ) in cervical cancer. Method:The physical status of HPV16 E2/E7 were detected by Multiplex PCR ( Polymerase chain reaction) in 42 patients with cervical cancer,55 patients with CIN and 20 patients with normal cervix tissues. And immunohistochemistry S-P assay was used to detect the expression of FHIT before operation. Results:The total positive expression rate of FHIT was 57. 26%. The positive expression rates of FHIT were respectively 85 . 00%、80 . 00%、75 . 00%、60 . 00% and 26. 19% in normal cervix tissues,CINⅠ,CINⅡ,CINⅢand cervical cancer tissues. The differ-ence was statistically significant (χ2=7 . 335 , P=0 . 003 ) . The total integration rate of HPV16 was 81. 20% in 117 only HPV16 positive cervical specimens. The integration rate of HPV16 were respectively 60. 00%、66. 67%、75. 00%、95. 00% and 92. 86% in normal cervix tissues, CINⅠ,CINⅡ,CINⅢ and cervical cancer tissues. And its showd an increasing trend in differ-ent cervical lesions(χ2=5. 713,P=0. 003). Between the total integration of HPV16 and the to-tal descending expression of FHIT were obvious difference (χ2=11 . 989 , P=0 . 000 ) . Conclu-sion:There are close relationship of HPV16 genome integration correlates with the descending expression of FHIT in the progression of cervical lesion. |
| Keywords: | Human papillomavirus Fragile histidine triad Integration Cervical intra-epithelial neoplasia Cervical cancer |
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