| 树突状细胞负载的exosome疫苗抗胶质瘤的实验研究 |
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| 引用本文： | 卜宁,吴海琴,孙秉中.树突状细胞负载的exosome疫苗抗胶质瘤的实验研究[J].现代肿瘤医学,2010,18(9). |
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| 作者姓名： | 卜宁 吴海琴 孙秉中 |
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| 作者单位： | 1. 西安交通大学医学院第二附属医院神经内科,陕西,西安,710004
2. 第四军医大学西京医院血液科,陕西,西安,710032 |
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| 基金项目： | 西安交通大学校长基金 |
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| 摘 要： | 目的:旨在分离胶质瘤细胞释放的exosomes,致敏外周血树突状细胞(dendritic cell,DCs),观察其对细胞毒性T淋巴细胞(cytotoxic T lymphocytcs,CTLs)的激活效应.方法:离心超滤和蔗糖密度梯度离心法分离胶质瘤细胞释放的exosomes,固相免疫电镜法(SPIEM)制备exosomes的MACE-1及ICAM-1免疫电镜标本.常规方法从外周血单个核细胞诱导DCs并分离T细胞,将胶质瘤细胞来源的exosomes冲击或未冲击的DCs与T细胞共培养.MTT比色法检测体外细胞毒活性.结果:胶质瘤细胞分泌的exosomes为直径50-100nm的膜性微囊,经抗MAGE-1、ICAM-1抗体-胶体金免疫电镜标记,囊外膜可见点状、颗粒状电子致密物沉积.培养后DCs的表面标志物CD1a、HLA-DR、CD83、CD86的表达率较培养前明显升高,差异有显著性(P＜0.05).exosomcs致敏的外周血DCs激活CTLs的能力显著高于肿瘤冻融抗原致敏的DCs组,在效靶比为50:1时,两组CTLs对胶质瘤细胞的杀伤率为70.4%±4.1%vs 30.1%±2.8%,(P＜0.05).结论:胶质瘤细胞分泌的exosomes负载外周血DCs后活化CTLs,发挥抗肿瘤活性,可以作为一种有效的抗胶质瘤免疫治疗策略和方法.
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| 关 键 词： | 树突状细胞 胶质瘤 |
The Experiment on the Anti- glioma Effects of Exosome Loaded Dendritic Cells Vaccine |
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| BU Ning,WU Hai-qin,SUN Bing-zhong.The Experiment on the Anti- glioma Effects of Exosome Loaded Dendritic Cells Vaccine[J].Journal of Modern Oncology,2010,18(9). |
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| Authors: | BU Ning WU Hai-qin SUN Bing-zhong |
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| Institution: | BU Ning1,WU Hai-qin1,SUN Bing-zhong21Department of Neurology,The Second Affiliated Hospital,Xi'an Jiaotong University,Xi'an 710004,China,2Department of Hematology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China. |
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| Abstract: | Objective:To observe the activation effect on cytotoxic T lymphocytes (CTLs) by dendritic cells (DCs) pulsed with exosomes derived from glioma cells.Methods:Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation.The expression of MAGE-1 and ICAM-1 were investigated by solid phase immunoelectron microscopic method (SPIEM).DCs and T cells were prepared from peripheral mononuclear cells by conventional methods.The DCs pulsed with or without glioma-derived exosomes wer... |
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| Keywords: | exosome |
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