赤芍中护肝活性物质的筛选与鉴定

目的:建立中药潜在护肝活性物质快速筛选新方法,辨识赤芍内具有护肝活性的化学成分。方法:在半乳糖胺HepG2细胞损伤模型上,采用二乙酸荧光素荧光标记法和MTT法筛选赤芍中具有护肝活性的组分,使用色谱-质谱联用技术分析其化学组成,并对筛选出的赤芍护肝活性成分进行实验验证。结果:从赤芍中筛选发现具有明显护肝活性的化学组分3个,并分析鉴定出芍药苷、棕榈酸乙酯和亚油酸乙酯3个成分。验证实验表明,这3个化合物对HepG2细胞均具有较明显的保护作用。结论:赤芍所含的芍药苷、棕榈酸乙酯和亚油酸乙酯具有潜在的护肝活性。

Screening and identifying of hepatoprotective compounds in Paeoniae Radix Rubra

Objective: To establish a new rapid method to screen potential hepatoprotective compounds from traditional Chinese medicine, and identify the hepatoprotective compounds in Paeoniae Radix Rubra. Method: Fluorescein diacetate labelled and MTT assay were applied for screening the hepatoprotective fractions on HepG2 cells exposed to galactosamine. The active fractions were analyzed by chromatography coupled with mass spectrometry. Finally, the hepatoprotective effects of the identified compounds were validated by hepatoprotective assay. Result: Three hepatoprotective fractions were founded, in which three compounds were identified as paeoniflorin, ethyl palmitate and ethyl linoleate. Validation results indicated that all the three compounds can attenuate the galactosamine induced injury on HepG2 cells. Conclusion: Paeoniflorin, ethyl palmitate and ethyl linoleate from paeoniae radix rubra showed potential hepatoprotective activity.