| LRP16蛋白在3T3-L1前脂肪细胞诱导分化中表达量的变化 |
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| 引用本文： | 马晓莉,母义明,金苗苗,汪保安. LRP16蛋白在3T3-L1前脂肪细胞诱导分化中表达量的变化[J]. 军医进修学院学报, 2011, 32(5): 486-488 |
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| 作者姓名： | 马晓莉 母义明 金苗苗 汪保安 |
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| 作者单位： | 1. 解放军总医院,内分泌科,北京,100853
2. 长治医学院附属和济医院,山西长治,046000 |
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| 基金项目： | 国家"973"重点基础研究发展规划基金 |
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| 摘 要： | 目的 LRP16蛋白在3T3-L1前脂肪细胞诱导分化过程中表达量的变化及胰岛素对脂肪细胞LRP16蛋白表达的调控。方法经典脂肪细胞诱导方案诱导前脂肪细胞为成熟脂肪细胞,每天（0-8d）均留取蛋白标本。不同浓度（0、1、10、100nmol/L）胰岛素刺激脂肪细胞24h,分别留取蛋白标本。Western-Blot方法检测样本中LRP16蛋白的表达量。结果 LRP16蛋白在前脂肪细胞无表达或极低表达,从诱导的第3、4天开始表达,迅速升至高峰,此后维持在高表达水平。LRP16蛋白在第6-8天之间表达水平无统计学差异（P〉0.05）,其余各时段间表达水平均有显著差异（P〈0.01）。不同浓度胰岛素刺激脂肪细胞24h,细胞中LRP16蛋白表达量与胰岛素浓度呈负相关（P〈0.01）。结论 LRP16蛋白随着前脂肪细胞分化的开启,表达量从无到有逐渐增加至高峰,并一直维持在高表达水平;胰岛素（INS）负调控脂肪细胞LRP16蛋白的表达。
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| 关 键 词： | LRP16蛋白 3T3-L1细胞 胰岛素 |
Expression of LRP16 protein during 3T3-L1 preadipocyte differentiation |
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| MA Xiao-li,MU Yi-ming,JIN Miao-miao,WANG Bao-an. Expression of LRP16 protein during 3T3-L1 preadipocyte differentiation[J]. Academic Journal of Pla Postgraduate Medical School, 2011, 32(5): 486-488 |
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| Authors: | MA Xiao-li MU Yi-ming JIN Miao-miao WANG Bao-an |
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| Affiliation: | MA Xiao-li1,MU Yi-ming1,JIN Miao-miao2,WANG Bao-an11Department of Endocrinology,Chinese PLA General Hospital,Beijing 100853,China,2Affiliated Heji Hospital of Changzhi Medical College,Changzhi,Shanxi Province 046000 |
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| Abstract: | Objective To study the expression levels of LRP16 protein in 3T3-L1 preadipocyte differentiation and the effect of insulin on LRP16 protein expression in LRP16 cells.Methods 3T3-L1 preadipocytes were cultured in vitro and differentiated into matured adipocytes,then protein samples were collected everyday(0-8d).Insulin was added into the culture medium of adipocytes for 24h at different concentrations(0,1,10,100nmol/L),and then protein samples were collected.Expression levels of LRP16 protein in 3T3-L1 cells... |
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| Keywords: | LRP16 Protein 3T3-L1 Cell Insulin |
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