BDNF-GFP转染神经干细胞对脊髓损伤大鼠BDNF表达的影响

目的探讨脑源性神经营养因子（Brain-Derived Neurotrophic Factor,BDNF）和绿色荧光蛋白（Green Fluorescent Protein,GFP）转染后神经干细胞（Neural Stem Cells,NSCs）移植对脊髓损伤大鼠BDNF表达的影响。方法以携带BDNF-GFP基因的腺病毒转染NSCs,免疫组化及Western blot检测转染后NSCs BDNF表达。40只Wistar大鼠中假手术组8只,32只大鼠制成T9左侧横断模型,并随机分成四组：BDNF和GFP修饰的N-SCs移植组、GFP修饰的NSCs移植组、单纯NSCs移植组和模型组。在各NSCs移植组,脊髓损伤后向横断处显微注射等体积细胞,模型组在相同的部位注射等体积的PBS。实时定量PCR检测各实验组BDNF表达情况。结果免疫组化显示BDNF-GFP转染的NSCs可表达BDNF（黄色荧光）。Western blot结果显示BDNF-GFP转染的NSCs可表达相对分子质量约为41kU的融合蛋白条带。NSCs移植可使BDNF的表达量明显增高（P〈0.05或P〈0.01）,以BDNF-GFP转染的NSCs移植组BDNF表达量最高（P〈0.01）。结论 BDNF-GFP转染后NSCs可在脊髓半切模型中存活并高表达具有生物活性的BDNF。

Effect of brain-derived neurotrophic factor and green fluorescent protein gene-transfected neural stem cells transplantation on brain-derived neurotrophic factor expression in rats with spinal cord injury

Objective To study the effect of brain-derived neurotrophic factor(BDNF) and green fluorescent protein(GFP)-transfected neural stem cells(NSCs) transplantation on expression of BDNF in rats with spinal cord injury.Methods NSCs were transfected with adenovirus vector bearing BDNF and GFP.Expression of BDNF in BDNF and GFP-transfected NSCs was detected by immunohistochemistry and Western blot,respectively.Of the 40 healthy Wistar rats,8 were selected as a sham-operation group,32 served as a T9 left hemisectio...