| microRNA调控NK细胞KIR3DL1表达初探 |
| |
| 引用本文： | 李晓红,吴晓雄,达万明,李猛,蔡博,于力,高春记. microRNA调控NK细胞KIR3DL1表达初探[J]. 军医进修学院学报, 2011, 32(5): 483-485,503 |
| |
| 作者姓名： | 李晓红 吴晓雄 达万明 李猛 蔡博 于力 高春记 |
| |
| 作者单位： | 1. 解放军总医院第一附属医院,血液科,北京,100048
2. 解放军总医院血液科,北京,100853 |
| |
| 摘 要： | 目的初步探寻人外周血自然杀伤细胞（Nature Killer,NK）杀伤细胞免疫球蛋白样受体（Killer Cell Immnoglobulin-Like Receptor,KIR3DL1）表达可能存在的microRNA（miR）调控机制。方法利用生物信息学方法,从miR信息库中筛选出可能与KIR3DL1相关的miRs,构建含KIR3DL1 3’非翻译区（UTR）的PGL3质粒,分别将含相应miR的PcDNA3.0质粒与前者共转染293T细胞,通过荧光素酶报告实验及之后的突变实验筛选出可能调控KIR3DL1的miR。结果通过TARGET SCAN信息库筛选了miR-146b等10个miR;转染miR-146b后,荧光素酶活性下降最多（61.3%）,突变其KIR3DL1 3’UTR靶位点后荧光素酶活性恢复（91.4%）。结论 miR-146b可与KIR3DL1’UTR在靶位点特异结合,很可能参与KIR3DL1表达的调控。
|
| 关 键 词： | microRNA 自然杀伤细胞 杀伤细胞免疫球蛋白样受体 荧光素酶报告实验 |
microRNA regulates KIR3DL1 expression in natural killer cells |
| |
| LI Xiao-hong,WU Xiao-xiong,DA Wan-ming,LI Meng,CAI Bo,YU Li,GAO Chun-ji. microRNA regulates KIR3DL1 expression in natural killer cells[J]. Academic Journal of Pla Postgraduate Medical School, 2011, 32(5): 483-485,503 |
| |
| Authors: | LI Xiao-hong WU Xiao-xiong DA Wan-ming LI Meng CAI Bo YU Li GAO Chun-ji |
| |
| Affiliation: | LI Xiao-hong1,WU Xiao-xiong1,DA Wan-ming2,LI Meng2,CAI Bo2,YU Li2,GAO Chun-ji2 1Department of Hematology,The First Affiliated Hospital,Chinese PLA General Hospital,Beijing 100048,China,2Department of Hematology,Beijing 100853 |
| |
| Abstract: | Objective To study the potential mechanism of microRNA(miR)underlying the regulation of KIR3DL1 expression in human peripheral blood derived natural killer(NK) cells.Methods KIR3DL1-associated miR was searched from miR database using bioinformatics techniques.PGL3 plasmids containing KIR3DL13'UTR were constructed.PcDNA3.0 containing corresponding miR and KIR3DL13'UTR were co-transfected into 293T cells.miR that may regulate KIR3DL1 expression before and after mutation was detected by luciferase reporter ass... |
| |
| Keywords: | microRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
