改良小鼠尾静脉注射器联合微量注射泵在Micro-CT碘海醇增强造影中的应用

目的探讨本研究组改良的穿刺设备及其他类型的穿刺设备在基于碘海醇的小鼠Micro-CT腹部增强造影中的效果。方法 100只昆明小鼠尾部热浴后,使用小动物麻醉机进行麻醉,并监测生命体征。应用5组设备行尾静脉穿刺:1常规1ml注射器及2.5#诺和锐针头;2常规1ml注射器及4.5#针头;3常规输液器7#针头;4常规输液器5#针头;5改良4.5#针头及输液连接装置,评价5组的优缺点并记录穿刺成功率。经Micro-CT扫描,记录图像采集率以及扫描后小鼠死亡率。结果 5组设备穿刺成功率、图像采集成功率以及小鼠死亡率均有差异(P<0.05)。综合比较各组成功率和小鼠死亡率,第5组为最优,能较好的对图像进行采集并且最大程度地减少小鼠的死亡。结论改良的尾静脉穿刺设备联合微量注射泵及小动物麻醉机的综合方式,能显著提高尾静脉穿刺和图像采集成功率以及降低小鼠死亡率,很好地应用于Micro-CT增强造影中,降低了实验动物成本。

Application of Improved Experimental Techniques of Caudal Intravenous Injection in Micro-CT Iohexol Contrast Enhancement

Objective To explore the effect of improved injection equipments on successful caudal intravenous injection with iohexol in contrast enhancement Micro-CT. Methods One hundred KM mice, were randomly divided into 5 groups with 20 in each group. The five groups were: 2.5^# Aspart needle; 4.5^# syringe needle; Infuser with 7^#needle; Infuser with 5^#needle; Infuser with 4.5^#needle. A total of 100 mice were anesthetized by small animal anesthesia machine and vital signs were monitored allowing for simultaneous cardiac and respiratory gating. And then Tail intravenous injection of contrast agent was performed using different devices (five groups) .The advantages and disadvantages of five equipments was eraluated and the success rate of puncture was recorded.Small animal was scaned Using Micro-CT. Image acquisition rate and mortality of mice after the end of the scan was recorded. Results The success rate of caudal intravenous injection and the image acquisition rate and the death rate of mice was significantly different among five groups(P<0.05).Of the above five groups, the fifth group best, which was accurate and efficient and can result in a significant reduction in deaths and made it easier for researcher to control the contrast agent injection rate and dose. Conclusion Application of improved puncture technique combined with micro injection pump, can greatly increase the success rate which will be applied to the Micro-CT iohexol contrast enhancement.