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| IL-8对血管内皮细胞紧密连接的影响 | |
| 引用本文： | 俞燚,刘肖珩,沈阳,高亭,高敏,马云龙,付强,康敏,赵健民. IL-8对血管内皮细胞紧密连接的影响[J]. 国际生物医学工程杂志, 2011, 34(6): 336-339,I0009. DOI: 10.3760/cma.j.issn.1673-4181.2011.06.004 |
| 作者姓名： | 俞燚 刘肖珩 沈阳 高亭 高敏 马云龙 付强 康敏 赵健民 |
| 作者单位： | 1. 四川大学华西基础医学与法医学院生物工程研究室, 成都,610041 2. 西藏大学医学院, 拉萨,850002 |
| 基金项目： | 国家自然科学基金资助项目，四川省青年基金资助项目，教育部新世纪优秀人才支持计划资助项目 |
| 摘 要： | 目的 研究白细胞介素-8(IL-8)对血管内皮细胞紧密连接的影响.方法 采用免疫荧光染色检测经不同浓度和时间IL-8处理后EA.hy926细胞的3种紧密连接蛋白occludin、claudin-5和ZO-1的形态和分布；逆转录PCR(RT-PCR)检测3种蛋白mRNA的表达水平.结果 IL-8可改变内皮细胞紧密连接蛋白... |
| 关 键 词： | 白细胞介素-8 血管内皮细胞 紧密连接 |
Effects of IL-8 on the tight junction of vascular endothelial cells |
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| YU Yi,LIU Xiao-heng,SHEN Yang,GAO Ting,GAO Min,MA Yun-long,FU Qiang,KANG Min,ZHAO Jian-min. Effects of IL-8 on the tight junction of vascular endothelial cells[J]. International Journal of Biomedical Engineering, 2011, 34(6): 336-339,I0009. DOI: 10.3760/cma.j.issn.1673-4181.2011.06.004 | |
| Authors: | YU Yi LIU Xiao-heng SHEN Yang GAO Ting GAO Min MA Yun-long FU Qiang KANG Min ZHAO Jian-min |
| Affiliation: | . (Institute of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China) |
| Abstract: | Objective To investigate the influence of IL-8 on the tight junction of vascular endothelial cells.Methods Immunofluorescence was used to observe the modality and the distribution of three tight junction proteins (occludin,claudin-5 and ZO-1) of the EA.hy926 cells treated with IL-8 under different concentrations and different times.RT-PCR was used to measure the mRNA expression of these three proteins.Results The results demonstrated that IL-8 could change the distribution of occludin,claudin-5 and ZO-1 in EA.hy926 cells,and the mRNA expression of occludin,claudin-5 and ZO-1 decreased with the increase of IL-8 concentration and treated time.Conclusion The effects of IL-8 on the distribution and the expression of occludin,claudin-5 and ZO-1 are dose and time-dependent. |
| Keywords: | Interleukin-8 Vascular endothelial cell Tight junction |
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