二甲胺四环素对大鼠脊髓损伤后iNOS表达及细胞凋亡的影响

目的探讨大鼠脊髓损伤后应用二甲胺四环素(minocycline)对诱导型一氧化氮合酶(iNOS)表达及细胞凋亡的影响.方法成年大鼠随机分为脊髓损伤后应用二甲胺四环素的治疗组(A组)和应用生理盐水对照组(B组),损伤后不同时间点取材,用HE染色观察损伤脊髓组织病理变化;用免疫组化染色检测iN-OS的表达变化;原位末端标记法(TUNEL法)标记凋亡细胞;采用开放场地试验BBB评分和斜板试验,评价神经功能.结果HE染色镜检发现脊髓组织病理学改变A组明显轻于B组.A、B两组均发现凋亡细胞及iNOS表达,神经细胞凋亡指数及iNOS表达均为B组＞A组(P＜0.05).治疗组神经功能改善优于B组.结论二甲胺四环素能抑制大鼠脊髓损伤后iNOS表达及细胞凋亡.

Effect of minocycline on expression of iNOS and apoptosis after spinal cord injury in rats

[Objective] To investigate the effect of minocycline on expression of iNOS and apoptosis after spinal cord injury(SCI)in rats.[Methods] Adult SD rats were divided at random into two groups:with pressure to cause spinal cord injury and use minocycline group(group A),and use normal saline group(group B).After operation the rats were sacrificed at 4 h,8 h,1 d,3 d,7 d,14 d and 21 d after surgery,and then the tissue sections were stained with hematoxylin and eosin(HE),immunohistochemistry and terminal deoxynucl-eotidyl transferase-mediated dUTP nick end labeling(TUNEL)method,and the injured spinal cord tissue was evaluated.[Results] The histopathology changes in spinal cord tissue observed with HE staining microscopic examination in group B was worse than that in group A.Expression of iNOS and apoptotic cells were observed in two groups,and the apoptotic index and expression of iNOS in group B was higher than that in group A(P <0.05).[Conclusion] This study demonstrated that minocycline can inhibit expression of iNOS and apoptosis after SCI in rats.