应用珊瑚／骨髓基质细胞／富血小板血浆组织工程骨修复兔颅骨缺损

目的：评价珊瑚／骨髓基质细胞（MSCs）／富血小板血浆（PRP）组织工程骨修复骨缺损的效果。方法：将兔MSCs悬液与同一供体来源的PRP混合后滴加到珊瑚团片上，形成珊瑚／MSCs／PRP复合物，用其修复MSCs和PRP来源兔颅骨极限缺损。自体颅骨或单纯珊瑚植入作为对照。术后6周和12周，通过组织学观察和组织形态测量分析．评价其骨修复效果。实验数据采用SPSS11．0软件包处理，两组之间差异采用t检验。结果：组织学观察显示，珊瑚／MSCs／PRP组，术后第6周，在整个缺损区珊瑚表面及其孔洞内有大量新骨形成；第12周，缺损区珊瑚完全降解吸收，被成熟的板层骨取代，缺损表现为完全的骨修复.图像分析显示：术后第6周和第12周，珊瑚／MSCs／PRP组的成骨量显著多于珊瑚组（P〈0．01）；术后第12周，珊瑚／MSCs／PRP组成骨量与自体骨组接近（P〉0．05）。结论：应用珊瑚作为支架材料、MSCs作为种子细胞、PRP作为内源性生长因子构建的组织工程骨修复骨缺损效果良好，与自体骨移植相近．是较理想的骨移植替代材料．

Treatment of rabbit calvarial defects with bone marrow stromal cells in combination with platelet-rich plasma in porous coral

PURPOSE： To evaluate local bone formation following surgical implantation of MSCs in combination with PRP in porous coral using a critical-size rabbit ealvarial defect model. METHODS： Natural coral disks with diameter of 15 mm anti thickness of 2ram were used in this study. A suspension of 3.3×10^7 MSCs in 300μL medium were dissolved in 300μL of PRP. The mixture of MSCs and PRP was spread on a coral disc. The construct was implanted into a critical-size 15mm rabbit calvariial defect. Coral disc alone or autograft was used as control. The bone formation was investigated with histological observation and histomorphometric analysis 6 and 12 weeks after operation. Significance was determined by Student＇s t test using SPSS 11.0 software package, We took P〈0.05 to be statistically significant. RESULTS： Six weeks after grafting, new bone was distributed throughout the coral scaffolds in specimens from the experimental group, and new bone appeared only in the periphery region of the coral scaffold from the coral group. Histomorphometric analysis revealed a significantly higher bone area in the experimental group than in the coral group （/：＇〈0.05）. Twelve weeks after grafting, the bone defects of the experimental group were repaired fully with bone, while those of the coral group were repaired partly with bone and fibrous tissue was evident in the central region of the defects. The experimental group had evident advantage over the coral group in term of bone regeneration （P〈0.01）. There was no statistically significant difference in bone formation between the experimental group and autograft group at 12-week post-surgery （P〉0.05）. CONCLUSION：Combination of MSCs with PRP in porous coral could enhance the bone healing considerably.