Preparation of midsagittal semithin Epon sections of the entire secretory ameloblast population in the maxillary rat incisor

Abstract – To study cytological changes in the migrating cell layer of the ameloblasts in the rat incisor with semithin Epon sections the ideal section plane would consequently be along the midsagittal plane of the incisor. However, the small dimensions and the complex three-dimensional structure of the apical part of the odontogenic organ covered by alveolar bone make it difficult to orientate and position sections in the midsagittal plane. Serial sectioning of the apical half of the maxillary incisor in the horizontal and sagittal planes clarified the relation of the midsagittal plane of the odontogenic organ to the alveolar bone, and the changes of the sectional profile of the odontogenic organ as a function of its position on the transverse axis of the incisor. From this information a method was designed whereby the two axes of the midagittal plane were marked directly on the block surface before sectioning, and the position of sections in the midsagittal zone of the ameloblasts standardized.