| 大蒜素二烯丙基三硫醚微乳的制备及其对肿瘤细胞增殖与迁移的影响 |
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| 引用本文： | 傅荣萍,刘玉萍,张毕桥,陈云艳,蒋祥亮,陈彦. 大蒜素二烯丙基三硫醚微乳的制备及其对肿瘤细胞增殖与迁移的影响[J]. 中草药, 2018, 49(23): 5541-5547 |
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| 作者姓名： | 傅荣萍 刘玉萍 张毕桥 陈云艳 蒋祥亮 陈彦 |
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| 作者单位： | 南京中医药大学附属中西医结合医院, 江苏 南京 210028;江苏省中医药研究院, 中药组分与微生态研究中心, 江苏 南京 210028,南京中医药大学附属中西医结合医院, 江苏 南京 210028;江苏省中医药研究院, 中药组分与微生态研究中心, 江苏 南京 210028,南京中医药大学附属中西医结合医院, 江苏 南京 210028,南京中医药大学附属中西医结合医院, 江苏 南京 210028;江苏省中医药研究院, 中药组分与微生态研究中心, 江苏 南京 210028,江苏省中医药研究院, 中药组分与微生态研究中心, 江苏 南京 210028,南京中医药大学附属中西医结合医院, 江苏 南京 210028;江苏省中医药研究院, 中药组分与微生态研究中心, 江苏 南京 210028 |
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| 基金项目： | 江苏省自然科学基金青年基金项目资助（BK20171097）；江苏省中医药局科技项目（YB2017033）；江苏省科教强卫医学重点人才项目（ZDRCA2016036） |
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| 摘 要： | 目的制备大蒜素二烯丙基三硫醚(DATS)微乳(DATS-MEs),评价该微乳载药系统对肿瘤细胞增殖和迁移能力的影响。方法以HS15为乳化剂,PEG400为助乳化剂,DATS作为油相,水滴定法制备DATS水包油微乳;采用四甲基偶氮唑蓝(MTT)法,评价DATS-MEs对小鼠Lewis肺癌(LLC)细胞及小鼠黑色素瘤B16BL6细胞体外增殖的影响;采用划痕实验评价DATS-MEs对LLC细胞及B16BL6细胞体外水平移行能力的影响。结果制备的DATS-MEs稳定,外观澄清透明,分散良好;粒径为(16.72±0.22)nm,多分散系数为0.03±0.01,Zeta电位为(-4.98±0.11)m V,DATS在微乳中的包封率为82.89%,载药量为6.28%,在水中的溶解度与裸药组相比增加了约2 367倍。10、30、50μmol/L的DATS-MEs与大蒜素裸药均能有效抑制LLC细胞和B16BL6细胞的增殖及迁移,并且具有剂量依赖性,其中DATS-MEs的药效强于DATS裸药。结论将DATS制成微乳,可提高其稳定性,可以有效解决DATS在水中溶解性差的问题,对LLC细胞及小鼠黑色素瘤B16BL6细胞具有较好的增殖抑制能力及迁移抑制能力,具有更好的抗肿瘤增殖及转移的潜力。
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| 关 键 词： | 大蒜素二烯丙基三硫醚 微乳 细胞增殖 细胞迁移 肺癌 黑色素瘤 |
| 收稿时间： | 2018-07-09 |
Preparation of diallyl trisulfide microemulsion and its effect on proliferation and migration of tumor cells |
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| FU Rong-ping,LIU Yu-ping,ZHANG Bi-qiao,CHEN Yun-yan,JIANG Xiang-liang and CHEN-Yan. Preparation of diallyl trisulfide microemulsion and its effect on proliferation and migration of tumor cells[J]. Chinese Traditional and Herbal Drugs, 2018, 49(23): 5541-5547 |
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| Authors: | FU Rong-ping LIU Yu-ping ZHANG Bi-qiao CHEN Yun-yan JIANG Xiang-liang CHEN-Yan |
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| Affiliation: | Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China;Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China;Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China;Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China,Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China and Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China;Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China |
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| Abstract: | |
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| Keywords: | diallyl trisulfide microemulsion cell proliferation cell migration lung cancer melanoma |
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