| 越鞠丸对Balb/c 小鼠和C57BL/6J 小鼠抗抑郁作用与前额叶场电位差异性分析 |
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| 引用本文： | 薛文达,聂瑞芳,周童,陈刚,王福顺,王薇.越鞠丸对Balb/c 小鼠和C57BL/6J 小鼠抗抑郁作用与前额叶场电位差异性分析[J].世界科学技术-中医药现代化,2018,20(9):1666-1672. |
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| 作者姓名： | 薛文达 聂瑞芳 周童 陈刚 王福顺 王薇 |
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| 作者单位： | 南京中医药大学基础医学院转化系统生物学和神经科学研究中心/中医脑病重点实验室 南京 210023;南通大学神经再生协同创新中心 南通 226001,南京中医药大学基础医学院转化系统生物学和神经科学研究中心/中医脑病重点实验室 南京 210023;南京中医药大学医学与生命科学学院 南京 210023,南京中医药大学基础医学院转化系统生物学和神经科学研究中心/中医脑病重点实验室 南京 210023;南京中医药大学医学与生命科学学院 南京 210023,南京中医药大学基础医学院转化系统生物学和神经科学研究中心/中医脑病重点实验室 南京 210023,南京中医药大学心理学院 南京210023,南京中医药大学基础医学院转化系统生物学和神经科学研究中心/中医脑病重点实验室 南京 210023;南京中医药大学心理学院 南京210023;南通大学神经再生协同创新中心 南通 226001 |
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| 基金项目： | 国家自然科学基金委青年基金项目（81803748）：VIPR2介导越鞠丸快速抗抑郁机制研究，负责人：薛文达；国家自然科学基金委青年基金项目（81603497）：基于ChIP-Seq的CREB介导越鞠丸快速抗抑郁作用分子机制研究，负责人：王薇；江苏省科技厅江苏省自然科学基金青年基金项目（BK20161040）：DISC1介导产后抑郁发生的机制研究，负责人：王薇；江苏省教育厅苏省高校自然科学基金面上基金项目（18KJB360009）：越鞠丸增强海马神经新生产生抗抑郁作用机制研究，负责人：王薇。 |
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| 摘 要： | 目的：比较Balb/c小鼠和C57BL/6J小鼠中越鞠丸抗抑郁作用的差异并分析其对小鼠前额皮层（prefontal cortex，PFC）突触传递的影响。方法：成年健康的雄性Balb/c小鼠和C57BL/6J小鼠随机分为对照组和越鞠丸组，给药30 min后进行强迫游泳测试（forced swimming test，FST），运用电生理实验（electrophysiologicalexperiment）检测小鼠前额皮层的场电位（field excitatory postsynaptic potential，fEPSP）及长时程增强（long termpotentiation，LTP）。结果：强迫游泳测试中，Balb/c小鼠的越鞠丸组不动时间较对照组明显降低（P < 0.01），C57BL/6J小鼠的的越鞠丸组和对照组在不动时间上没有明显差异（P > 0.05）。电生理实验中，Balb/c小鼠的越鞠丸组与对照组相比，场电位斜率百分比显著升高（P < 0.01），而C57BL/6J小鼠的越鞠丸组和对照组的场电位斜率百分比无显著性差异（P > 0.05）；Balb/c小鼠的越鞠丸组与对照组相比，长时程增强显著升高（P < 0.01），而C57BL/6J小鼠的越鞠丸组和对照组的长时程增强无显著性差异（P > 0.05）。结论：越鞠丸可能通过增加Balb/c小鼠前额皮层的场电位及长时程增强以提高突触传递效能，从而产生抗抑郁作用。
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| 关 键 词： | 越鞠丸 抗抑郁作用 前额皮层 场电位 长时程增强 |
Analysis of Differences of Antidepressant Effect of Yueju Pill between Balb/c Mice and C57BL/6J Mice |
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| Xue Wend,Nie Ruifang,Zhou Tong,Chen Gang,Wang Fushun and Wang Wei.Analysis of Differences of Antidepressant Effect of Yueju Pill between Balb/c Mice and C57BL/6J Mice[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2018,20(9):1666-1672. |
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| Authors: | Xue Wend Nie Ruifang Zhou Tong Chen Gang Wang Fushun and Wang Wei |
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| Institution: | Center for Translational Systems Biology and Neuroscience, Key Laboratory of Integrative Medicine for Brain Diseases, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China;Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China,Center for Translational Systems Biology and Neuroscience, Key Laboratory of Integrative Medicine for Brain Diseases, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China;School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China,Center for Translational Systems Biology and Neuroscience, Key Laboratory of Integrative Medicine for Brain Diseases, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China;School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China,Center for Translational Systems Biology and Neuroscience, Key Laboratory of Integrative Medicine for Brain Diseases, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023,School of Psychology, Nanjing University of Chinese Medicine, Nanjing 210023, China and Center for Translational Systems Biology and Neuroscience, Key Laboratory of Integrative Medicine for Brain Diseases, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023;School of Psychology, Nanjing University of Chinese Medicine, Nanjing 210023, China;Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China |
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| Abstract: | This paper aimed at comparing the differences of antidepressant effects of Yueju Pill between the Balb/c miceand C57BL/6J mice and analyzing the effects on synaptic transmission in mice prefrontal cortex. Healthy adult Balb/cmice and C57BL/6J mice were randomly divided into control group and Yueju Pill group. Control group was treated withsaline, Yueju Pill group was treated with single dose of Yueju Pill (13.5 g·kg- 1). The forced swimming test (FST) wasmeasured 30 min after administration. The field excitatory postsynaptic potential (fEPSP) and long-term potentiation(LTP) of mice prefrontal cortex were detected by the electrophysiological experiment. In FST, the immobility time of Balb/c mice in Yueju Pill group was significantly lower than that in control group (P < 0.01), and the immobility time of C57BL/6J mice showed no remarkable difference between Yueju Pill group and control group (P > 0.05). In electrophysiologicalexperiment, the percentage of slope of fEPSP in Balb/c mice in Yueju Pill group was significantly higher than that incontrol group (P < 0.01), while there was no significantly difference in C57BL/6J mice between Yueju Pill group andcontrol group (P > 0.05). The LTP of Balb/c mice in Yueju Pill group was significantly increased than that in controlgroup (P < 0.01), while there was no significantly difference in LTP of C57BL/6J mice between Yueju Pill group andcontrol group (P > 0.05). Yueju Pill may display rapid antidepressant effect via increasing fEPSP and LTP in prefrontalcortex of Balb/c mice and then enhancing the synaptic transmission. |
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| Keywords: | Yueju Pills antidepressant prefrontal cortex fEPSP LTP |
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