| 核定位序列修饰的二氧化钛纳米颗粒对胶质瘤U251细胞的放射增敏作用 |
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| 引用本文： | 商军,解婷,殷娜瑞,陈飞,杜杰,张昊文,俞家华,刘芬菊. 核定位序列修饰的二氧化钛纳米颗粒对胶质瘤U251细胞的放射增敏作用[J]. 中华放射医学与防护杂志, 2018, 38(11): 801-806 |
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| 作者姓名： | 商军 解婷 殷娜瑞 陈飞 杜杰 张昊文 俞家华 刘芬菊 |
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| 作者单位： | 215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室,215123 苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室 江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室 |
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| 基金项目： | 国家自然科学基金（31270897，81271682，31770913，81771947）；江苏省高校优势学科（PAPD） |
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| 摘 要： | ![]()
目的 研究核定位序列（nuclear localization sequence，NLS）修饰的二氧化钛（titanium dioxide，TiO2）纳米颗粒对胶质瘤U251细胞放射敏感性的作用。方法 合成并表征具有细胞核靶向性的TiO2-NLS纳米颗粒，分别用纳米颗粒和电离辐射单独或联合处理U251细胞，流式细胞仪检测活性氧（ROS）含量和细胞凋亡率，γ-H2AX焦点（foci）染色检测DNA损伤修复的情况，细胞克隆形成实验检测细胞放射敏感性的改变。结果 NLS修饰可促进TiO2纳米颗粒进入U251细胞核，与未修饰的TiO2纳米颗粒相比，TiO2-NLS纳米颗粒可促进电离辐射诱导的细胞凋亡（t=8.96，P<0.05），细胞克隆实验显示，TiO2-BSA纳米颗粒组的放射增敏比为1.18，TiO2-NLS纳米颗粒处理组的放射增敏比为1.29，两组比较，差异有统计学意义（t=14.72，P<0.05）。结论 具有细胞核靶向性的TiO2纳米颗粒能够增强胶质瘤U251细胞的放射敏感性。
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| 关 键 词： | 二氧化钛纳米颗粒 核定位序列 放射增敏 胶质瘤细胞 |
| 收稿时间： | 2018-06-01 |
Titanium dioxide nanoparticles coupled with nuclear localization sequence increases the radiosensitivity of U251 glioma cells |
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| Shang Jun,Xie Ting,Yin Narui,Chen Fei,Du Jie,Zhang Haowen,Yu Jiahua and Liu Fenju. Titanium dioxide nanoparticles coupled with nuclear localization sequence increases the radiosensitivity of U251 glioma cells[J]. Chinese Journal of Radiological Medicine and Protection, 2018, 38(11): 801-806 |
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| Authors: | Shang Jun Xie Ting Yin Narui Chen Fei Du Jie Zhang Haowen Yu Jiahua Liu Fenju |
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| Affiliation: | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China,School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China,School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China,School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China,School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China,School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China,School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China and School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China |
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| Abstract: | ![]()
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| Keywords: | Titanium dioxide nanoparticles Nuclear localization sequence Radiosensitivity Glioma cells |
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