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| 高效液相色谱法测定二十七味定坤丸中橙皮苷的含量 | |
| 引用本文： | 王湘妍. 高效液相色谱法测定二十七味定坤丸中橙皮苷的含量[J]. 中国中医药现代远程教育, 2013, 11(1): 150-151 |
| 作者姓名： | 王湘妍 |
| 作者单位： | 河南省医药学校 |
| 摘 要： | 目的建立高效液相色谱法测定二十七味定坤丸中橙皮苷的含量。方法Agela Promosil C18（250mm×4mm，5μm）分析柱，流动相为乙腈-水-冰醋酸（15：84：1），流速为1．0mL·min^-1，检测波长为284nm。结果橙皮苷线性范围为0．255～3．066μg（r=09998）；平均回收率为98．82％，RSD=0．42％（n=6）。结论本法简便、准确可靠，可用于该药的质量控制。 |
| 关 键 词： | 高效液相色谱 二十七味定坤丸 橙皮苷 |
HPLC Method for the Determination of Twenty-seven Kinds of Dingkun Pills in the Content of Hesperidin |
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| Wang Xiangyan. HPLC Method for the Determination of Twenty-seven Kinds of Dingkun Pills in the Content of Hesperidin[J]. Chinese Medicine Modern Distance Education of China, 2013, 11(1): 150-151 | |
| Authors: | Wang Xiangyan |
| Affiliation: | Wang Xiangyan ( Henan Pharmaceutical School,Kaifeng 475000,China) |
| Abstract: | Objective to establish a HPLC method for the determination of twenty-seven kinds of Dingkun pills in the content of hesperidin. Methods Agela Promosil C18 （250 mm × 4mm, 5μm） column, the mobile phase consisted of acetonitrile-water-aeetie acid （15 ： 84 ： 1）, the flow rate was 1 mL · min^-1, the detection wavelength was284 nm. results： hesperidin was linear in the range of 0. 255μg-3. 066μg （ r = 0. 9998） ; the average recovery rate as of 98. 82%, RSD= 0. 42% （ n = 6）. Conclusion. this method is simple, accurate and reliable, and can be used for the quality control of drugs. |
| Keywords: | High performance liquid chromatography Twenty - seven taste of Dingkun pills Hesperidin |
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